Immunoprecipitation(IP) of MAPK1 by using monoclonal anti-MAPK1 antibodies (Negative control: IP without adding anti-MAPK1 antibody.). For each experiment, 500ul of DDK tagged MAPK1 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2 ug of anti-MAPK1 antibody and 20ul (0.1 mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.
MAPK1 / ERK2 Antibody (clone OTI6F8, Carrier-free)
Primary AntibodyLSBio Guarantee
| Antibody: | Mouse Monoclonal |
| Applications: | Flow Cytometry, Immunohistochemistry (general), Immunoprecipitation, Western Blot |
| Reactivity: | Dog, Human, Non-Human Primate, Rat |
| Format: | Lyophilized |
$522.00each
Catalog Number: LS-C784287-100
Catalog Number: LS-C784287
For bulk orders or custom formulations:
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- Description
- ERK2 antibody LS-C784287 is an unconjugated mouse monoclonal antibody to ERK2 (MAPK1) from human. It is reactive with human, rat, dog and other species. Validated for Flow, IHC, IP and WB.
- Application
- FC, IHC, IP, WB
- Reactivity
- Can, Hu, NHP, Rt
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Monoclonal Antibodies
- Alternative Names
- ERK | ERK-2 | ERK2 | MAP kinase 1 | MAPK 2 | MAPK2 | MAP kinase isoform p42 | MAPK 1 | MAPK1 | p41mapk | PRKM2 | MAP kinase 2 | p41 | p42-MAPK | PRKM1 | ERT1 | p38 | p40 | p42MAPK | Protein tyrosine kinase ERK2
- UniProt Number
- P28482
- NCBI GenBank
- NM_002745 | NP_002736.3
- SwissProt
- P28482
Recommended Dilution
| Application | Dilution |
|---|---|
| FC | 1:100 |
| IHC | 1:50 |
| IP | 4 µg/ml |
| WB | 1:1000 - 1:2000 |
Target
- Target
- Human MAPK1 / ERK2
- Antigen Species
- Human
- Gene Name
- MAPK1
Antibody
- Host Species
- Mouse
- Clonality
- Monoclonal
- Clone
- OTI6F8
- Isotype
- IgG2a
- Origin Species
- Human
Immunogen
- Immunogen
- Full length human recombinant protein of human MAPK1(NP_620407) produced in HEK293T cell.
- Immunogen Type
- Full-Length Recombinant Protein
Format
- Conjugate/Label
- Unconjugated
- Format
- Lyophilized
- Formulation
- PBS, pH 7.3, 8% Trehalose
- Preservative
- No
- Purification Method
- Affinity Purified
Storage & Handling
- Storage Conditions
- Store at -20°C. Avoid freeze-thaw cycles.
- Recommended Storage Buffer
- PBS