Western blot analysis of extracts from 293 cells, treated with TNF (20ng/ml, 15mins) and calyculin A (50nM, 15mins), using ASK1 (Phospho-Ser966) antibody.
MAP3K5 / ASK1 Antibody (phospho-Ser966)
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general), Western Blot |
| Reactivity: | Human, Mouse |
| Format: | Liquid |
$365.00each
Catalog Number: LS-C825226-100
Catalog Number: LS-C825226
For bulk orders or custom formulations:
Request a QuoteOverview
- Description
- ASK1 antibody LS-C825226 is an unconjugated rabbit polyclonal antibody to ASK1 (MAP3K5) (pSer966) from human. It is reactive with human and mouse. Validated for ELISA, IHC and WB.
- Application
- ELISA, IHC, WB
- Reactivity
- Hu, Ms
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- ASK-1 | ASK1 | MAP3K5 | MEKK5 | MEK kinase 5 | MEKK 5 | MAPK/ERK kinase kinase 5 | MAPKKK5 | MAP/ERK kinase kinase 5
- Recommended Dilution: ELISA
- 1:20000
- Recommended Dilution: IHC
- 1:50 - 1:100
- Recommended Dilution: WB
- 1:500 - 1:1000
- UniProt Number
- Q99683
- NCBI GenBank
- NM_005923 | NP_005914.1
- SwissProt
- Q99683
Target
- Target
- Human MAP3K5 / ASK1
- Antigen Species
- Human
- Epitope
- ASK1 (Phospho-Ser966) antibody detects endogenous levels of ASK1 only when phosphorylated at serine 966. (Human: Ser966; Mouse: Ser973)
- Gene Name
- MAP3K5
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Isotype
- IgG
- Origin Species
- Human
Immunogen
- Immunogen
- Synthesized phosphopeptide derived from human ASK1 around the phosphorylation site of serine 966 (S-I-SP-L-P).
- Immunogen Type
- Synthetic Peptide (Linear)
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- PBS (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
- Preservative
- Yes
- Purification Method
- Affinity Purified
Storage & Handling
- Storage Conditions
- Store at -20°C for up to 1 year.
- Recommended Storage Buffer
- PBS|150mM NaCl