Western blot analysis of extracts of HeLa cells, using MAP3K5 antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 90s.
MAP3K5 / ASK1 Antibody
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Western Blot |
| Reactivity: | Human |
| Format: | Liquid |
$281.00each
Catalog Number: LS-C747559-20
Catalog Number: LS-C747559
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- Description
- ASK1 antibody LS-C747559 is an unconjugated rabbit polyclonal antibody to human ASK1 (MAP3K5). Validated for WB.
- Application
- WB
- Reactivity
- Hu
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- ASK-1 | ASK1 | MAP3K5 | MEKK5 | MEK kinase 5 | MEKK 5 | MAPK/ERK kinase kinase 5 | MAPKKK5 | MAP/ERK kinase kinase 5
- Recommended Dilution: WB
- 1:500 - 1:2000
- UniProt Number
- Q99683
- NCBI GenBank
- NM_005923 | NP_005914.1
- SwissProt
- Q99683
Target
- Target
- Human MAP3K5 / ASK1
- Antigen Species
- Human
- Gene Name
- MAP3K5
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Isotype
- IgG
- Origin Species
- Human
Immunogen
- Immunogen
- A synthetic peptide corresponding to a sequence within amino acids 1300 to the C-Terminus of human MAP3K5 (NP_005914.1). HLNSSGTNTEDSELTDWLRVNGADEDTISRFLAEDYTLLDVLYYVTRDDLKCLRLRGGMLCTLWKAIIDFRNKQT
- Immunogen Type
- Synthetic Peptide (Linear)
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- PBS, pH 7.3, 0.02% Sodium Azide, 50% Glycerol
- Volume
- 20 Microliter
- Preservative
- Yes
- Purification Method
- Affinity Purified
Storage & Handling
- Storage Conditions
- Store at -20°C. Avoid freeze-thaw cycles.
- Recommended Storage Buffer
- PBS