Formalin-fixed paraffin-embedded tissue section of human reactive tonsil stained for ASK1 expression using Polyclonal Antibody to ASK1 at 1:2000. A and A1. Low and high magnification, respectively. ASK1 expression was seen in the vasculature (endothelial cells). Hematoxylin-Eosin counterstain.
MAP3K5 / ASK1 Antibody (aa1198-1216)
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Immunohistochemistry (general), Immunohistochemistry, Frozen, Immunohistochemistry, Paraffin, Immunoprecipitation, Western Blot |
| Reactivity: | Human |
| Format: | Liquid |
$394.00each
Catalog Number: LS-C148204-50
Catalog Number: LS-C148204
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- Description
- ASK1 antibody LS-C148204 is an unconjugated rabbit polyclonal antibody to human ASK1 (MAP3K5) (aa1198-1216). Validated for IHC, IP and WB.
- Application
- IHC, IHC-F, IHC-P, IP, WB
- Reactivity
- Hu
- Predicted Reactivity
- Non-Human Primate
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- ASK-1 | ASK1 | MAP3K5 | MEKK5 | MEK kinase 5 | MEKK 5 | MAPK/ERK kinase kinase 5 | MAPKKK5 | MAP/ERK kinase kinase 5
- Recommended Dilution: IHC-P
- 1:1000 - 1:5000
- Recommended Dilution: IP
- 1:50 - 1:200
- Recommended Dilution: WB
- 1:1000 - 1:2000
- UniProt Number
- Q99683
- NCBI GenBank
- NM_005923 | NP_005914.1
- SwissProt
- Q99683
Target
- Target
- Human MAP3K5 / ASK1
- Antigen Species
- Human
- Epitope
- ASK1.
- Gene Name
- MAP3K5
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
Immunogen
- Immunogen
- A synthetic peptide corresponding to amino acids 1198-1216(C-HEEQPSNQTVRRPQAVIED) of human Ask1, GenBank no. NP_005914.1. Human Ask1 is a 1374 amino acid protein. The peptide immunogen sequence is 100% conserved in Ask1 variant (BAD92330.1), a 1455 amino acid protein. Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Monkey, Galago (100%); Gibbon, Marmoset, Elephant, Guinea pig (95%); Dog, Rabbit (89%); Ferret, Panda, Cat, Opossum (84%); Mouse, Rat (79%).
- Immunogen Type
- Synthetic Peptide (Linear)
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- Antiserum, 0.05% sodium azide
- Volume
- 50 Microliter
- Preservative
- Yes
- Purification Method
- Unpurified (Serum / Ascites / Supernatant)
Storage & Handling
- Storage Conditions
- Store at -20°C. Aliquot to avoid freeze/thaw cycles.