Immunoprecipitation(IP) of MAP2K4 by using monoclonal anti-MAP2K4 antibodies (Negative control: IP without adding anti-MAP2K4 antibody.). For each experiment, 500ul of DDK tagged MAP2K4 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2 ug of anti-MAP2K4 antibody and 20ul (0.1 mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.
MAP2K4 / MKK4 Antibody (clone OTI4B4, Carrier-free)
Primary AntibodyLSBio Guarantee
| Antibody: | Mouse Monoclonal |
| Applications: | Flow Cytometry, Immunofluorescence, Immunohistochemistry (general), Immunoprecipitation, Western Blot |
| Reactivity: | Human |
| Format: | Lyophilized |
$522.00each
Catalog Number: LS-C784239-100
Catalog Number: LS-C784239
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- Description
- MKK4 antibody LS-C784239 is an unconjugated mouse monoclonal antibody to human MKK4 (MAP2K4). Validated for Flow, IF, IHC, IP and WB.
- Application
- FC, IF, IHC, IP, WB
- Reactivity
- Hu
- Intended Use
- RUO
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Monoclonal Antibodies
- Alternative Names
- JNK activating kinase 1 | JNK-activated kinase 1 | JNK-activating kinase 1 | MAPK/ERK kinase 4 | MAPKK4 | JNKK1 | PRKMK4 | SAPKK1 | SEK1 | MEK4 | MKK4 | SAPK/ERK kinase 1 | SERK1 | JNKK | MAP kinase kinase 4 | MAP2K4 | MAPKK 4 | MEK 4 | SAPK kinase 1 | SAPKK-1 | SKK1
- Recommended Dilution: FC
- 1:100
- Recommended Dilution: IF/ICC
- 1:100
- Recommended Dilution: IHC
- 1:150
- Recommended Dilution: IP
- 4 µg/ml
- Recommended Dilution: WB
- 1:2000
- UniProt Number
- P45985
- NCBI GenBank
- NM_003010, NP_003001.1
- SwissProt
- P45985
Target
- Target
- Human MAP2K4 / MKK4
- Antigen Species
- Hu
- Gene Name
- MAP2K4
Antibody
- Host Species
- Ms
- Clonality
- Monoclonal
- Clone
- OTI4B4
- Isotype
- IgG2a
- Origin Species
- Hu
Immunogen
- Immunogen
- Full-length protein expressed in 293T cell transfected with human MAP2K4 expression vector.
- Immunogen Type
- Cell
Format
- Conjugate/Label
- Unconj
- Format
- Lyophilized
- Formulation
- PBS, pH 7.3, 8% Trehalose
- Preservative
- No
- Purification Method
- Other
Storage & Handling
- Storage Conditions
- Store at -20°C. Avoid freeze-thaw cycles.
- Recommended Storage Buffer
- PBS