Immunofluorescence staining of MAP2K3 in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human MAP2K3 polyclonal antibody (dilution ratio 1:1000) at 4°C overnight. Then cells were stained with the Alexa Fluor 488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to cytoplasm and nucleus.
MAP2K3 / MEK3 / MKK3 Antibody (Preservative Free)
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Paraffin, Immunoprecipitation, Western Blot |
| Reactivity: | Human, Mouse |
| Format: | Liquid |
$309.00each
Catalog Number: LS-C815370-100
Catalog Number: LS-C815370
For bulk orders or custom formulations:
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- Description
- MKK3 antibody LS-C815370 is an unconjugated rabbit polyclonal antibody to MKK3 (MAP2K3 / MEK3) from human. It is reactive with human and mouse. Validated for ICC, IF, IHC, IP and WB.
- Application
- ICC, IF, IHC-P, IP, WB
- Reactivity
- Hu, Ms
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- MAP2K3 | MAPKK 3 | MAPKK3 | PRKMK3 | SAPK kinase 2 | SAPKK-2 | SAPKK2 | SKK2 | MAP kinase kinase 3 | MEK 3 | MEK3 | MKK3 | MAPK/ERK kinase 3
- Recommended Dilution: IF/ICC
- 1:300 - 1:10000
- Recommended Dilution: IHC-P
- 1:500 - 1:2000
- Recommended Dilution: WB
- 1:500 - 1:2000
- UniProt Number
- P46734
- NCBI GenBank
- NM_002756 | NP_002747.2
- SwissProt
- P46734
Target
- Target
- Human MAP2K3 / MEK3 / MKK3
- Antigen Species
- Human
- Epitope
- Human MEK3 / MKK3
- Gene Name
- MAP2K3
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Isotype
- IgG
- Origin Species
- Human
Immunogen
- Immunogen
- A synthetic peptide corresponding to the C-Terminus of Human MAP2K3/MKK3
- Immunogen Type
- Synthetic Peptide (Linear)
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- 0.2 µm filtered solution in PBS
- Volume
- 100 Microliter
- Preservative
- No
- Purification Method
- Protein A Affinity
Storage & Handling
- Storage Conditions
- Store at 2°C to 8°C for up to 1 month. Aliquot and store at -20°C to -80°C for up to 1 year. Avoid freeze/thaw cycles.
- Recommended Storage Buffer
- PBS