Mouse MAP2K1 was immunoprecipitated using: Lane A: 0.5 mg Jurkat Whole Cell Lysate. Lane B: 0.5 mg Hela Whole Cell Lysate. 1 uL anti-Mouse MAP2K1 rabbit polyclonal antibody and 15 ul of 50% Protein G agarose. Primary antibody: Anti-Mouse MAP2K1 rabbit polyclonal antibody, at 1:500 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the odssey technique. Performed under reducing conditions. Predicted band size: 43 kDa. Observed band size: 43 kDa.
MAP2K1 / MKK1 / MEK1 Antibody (Preservative Free)
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Enzyme-Linked Immunosorbent Assay, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Paraffin, Immunoprecipitation, Western Blot |
| Reactivity: | Mouse |
| Format: | Liquid |
$309.00each
Catalog Number: LS-C818588-100
Catalog Number: LS-C818588
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- Description
- MEK1 antibody LS-C818588 is an unconjugated rabbit polyclonal antibody to mouse MEK1 (MAP2K1 / MKK1). Validated for ELISA, ICC, IF, IHC, IP and WB.
- Application
- ELISA, ICC, IF, IHC-P, IP, WB
- Reactivity
- Ms
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- ERK activator kinase 1 | MAP2K1 | MAPK/ERK kinase 1 | MAPKK 1 | MKK1 | PRKMK1 | MEK 1 | MAP kinase kinase 1 | MAPKK1 | MEK1
- Recommended Dilution: ELISA
- 1:25000 - 1:50000
- Recommended Dilution: IF/ICC
- 1:1500 - 1:50000
- Recommended Dilution: IHC-P
- 1:2500 - 1:10000
- Recommended Dilution: WB
- 1:500 - 1:1000
- UniProt Number
- Q02750
- NCBI GenBank
- NM_002755 | NP_002746.1
- SwissProt
- Q02750
Target
- Target
- Mouse MAP2K1 / MKK1 / MEK1
- Antigen Species
- Mouse
- Epitope
- Mouse MEK1
- Gene Name
- MAP2K1
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Isotype
- IgG
- Origin Species
- Human
Immunogen
- Immunogen
- Recombinant Mouse MEK1 / MAP2K1 / MKK1 Protein
- Immunogen Type
- Recombinant Immunogen
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- 0.2 µm filtered solution in PBS
- Volume
- 100 Microliter
- Preservative
- No
- Purification Method
- Protein A Affinity
Storage & Handling
- Storage Conditions
- Store at 2°C to 8°C for up to 1 month. Aliquot and store at -20°C to -80°C for up to 1 year. Avoid freeze/thaw cycles.
- Recommended Storage Buffer
- PBS