Western blot of NIH 3T3 cell lysates showing specific immunolabeling of the ~45k MEK 1/2 protein phosphorylated at Ser218 and Ser222. The cells were either serum starved (Control) or incubated in the presence of serum (Serum). Immunolabeling of an additional band at ~95k was also observed.
MAP2K1 / MKK1 / MEK1 Antibody (phospho-Ser218/Ser222)
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Western Blot |
| Reactivity: | Bovine, Chicken, Dog, Human, Mouse, Rat, Xenopus |
| Format: | Liquid |
$479.00each
Catalog Number: LS-C186-100
Catalog Number: LS-C186
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- Description
- MEK1 antibody LS-C186 is an unconjugated rabbit polyclonal antibody to MEK1 (MAP2K1 / MKK1) (pSer218/Ser222) from human. It is reactive with human, mouse, rat and other species. Validated for WB.
- Application
- WB
- Reactivity
- Bov, Can, Ck, Hu, Ms, Rt, Xen
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- ERK activator kinase 1 | MAP2K1 | MAPK/ERK kinase 1 | MAPKK 1 | MKK1 | PRKMK1 | MEK 1 | MAP kinase kinase 1 | MAPKK1 | MEK1 | p-Ser218 | 222 MEK 1/2
- Recommended Dilution: WB
- 1:1000
- UniProt Number
- Q02750
- NCBI GenBank
- NM_002755 | NP_002746.1
- SwissProt
- Q02750
Target
- Target
- Human MAP2K1 / MKK1 / MEK1
- Antigen Species
- Human
- Epitope
- Specific for the ~45k MEK 1/2 protein phosphorylated at Ser218/222 in Western blots. Immunolabeling is blocked by the phosphopeptide used as the antigen but not by the corresponding dephosphopeptide
- Gene Name
- MAP2K1
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
Immunogen
- Immunogen
- A phosphopeptide corresponding to amino acid residues surrounding the phosphoSer218222 of human MEK 1/2. Note: The phosphosites in human MEK 1/2 are Ser218 and Ser222; the phosphosites in mouse MEK 1/2 are Ser217 and Ser221.
- Immunogen Type
- Peptide
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- 10 mM HEPES, pH 7.5, 150 mM NaCl, 0.1 mg/mL BSA, 50% Glycerol
- Volume
- 100 Microliter
- Preservative
- No
- Purification Method
- Affinity Purified
Storage & Handling
- Storage Conditions
- Short term: -20°C; Long term: -20°C.
- Recommended Storage Buffer
- 10 mM HEPES|150 mM NaCl