Western blot analysis of Human Lung carcinoma epithelial cell line (A549) lysate showing detection of ~43.4 kDa MEK1 protein using Rabbit Anti-MEK1 Polyclonal Antibody. Lane 1: Molecular Weight Ladder (MW). Lane 2: A459. Load: 10 µg. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-MEK1 Polyclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:5000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~43.4 kDa.
MAP2K1 / MKK1 / MEK1 Antibody
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Immunohistochemistry (general), Western Blot |
| Reactivity: | Human |
| Format: | Liquid |
$451.00each
Catalog Number: LS-C774756-100
Catalog Number: LS-C774756
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- Description
- MEK1 antibody LS-C774756 is an unconjugated rabbit polyclonal antibody to human MEK1 (MAP2K1 / MKK1). Validated for IHC and WB.
- Application
- IHC, WB
- Reactivity
- Hu
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- ERK activator kinase 1 | MAP2K1 | MAPK/ERK kinase 1 | MAPKK 1 | MKK1 | PRKMK1 | MEK 1 | MAP kinase kinase 1 | MAPKK1 | MEK1
- Recommended Dilution: IHC
- 1:50
- Recommended Dilution: WB
- 1:1000
- UniProt Number
- Q02750
- NCBI GenBank
- NM_002755 | NP_002746.1
- SwissProt
- Q02750
Target
- Target
- Human MAP2K1 / MKK1 / MEK1
- Antigen Species
- Human
- Epitope
- Detects 43.4 kDa.
- Gene Name
- MAP2K1
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Origin Species
- Human
Immunogen
- Immunogen
- Synthetic peptide from the mid-protein of Human MEK1 (aa. 250-350)
- Immunogen Type
- Synthetic Peptide (Linear)
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- PBS, pH 7.4, 0.09% Sodium Azide, 50% Glycerol
- Concentration
- 1 mg/mL
- Preservative
- Yes
- Purification Method
- Affinity Purified
Storage & Handling
- Storage Conditions
- Store at -20°C.
- Recommended Storage Buffer
- PBS