Immunofluorescence staining of MEK1 in NIH/3T3 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Mouse MEK1 monoclonal antibody (dilution ratio 1:60) at 4°C overnight. Then cells were stained with the Alexa Fluor 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to Cytoplasm.
MAP2K1 / MKK1 / MEK1 Antibody (clone 131, Preservative Free)
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Monoclonal |
| Applications: | Immunocytochemistry, Immunofluorescence, Western Blot |
| Reactivity: | Mouse |
| Format: | Liquid |
$309.00each
Catalog Number: LS-C819369-100
Catalog Number: LS-C819369
For bulk orders or custom formulations:
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- Description
- MEK1 antibody LS-C819369 is an unconjugated rabbit monoclonal antibody to mouse MEK1 (MAP2K1 / MKK1). Validated for ICC, IF and WB.
- Application
- ICC, IF, WB
- Reactivity
- Ms
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Monoclonal Antibodies
- Alternative Names
- ERK activator kinase 1 | MAP2K1 | MAPK/ERK kinase 1 | MAPKK 1 | MKK1 | PRKMK1 | MEK 1 | MAP kinase kinase 1 | MAPKK1 | MEK1
- Recommended Dilution: IF/ICC
- 1:20 - 1:100
- Recommended Dilution: WB
- 1:500 - 1:2000
- UniProt Number
- Q02750
- NCBI GenBank
- NM_002755 | NP_002746.1
- SwissProt
- Q02750
Target
- Target
- Mouse MAP2K1 / MKK1 / MEK1
- Antigen Species
- Mouse
- Epitope
- Mouse MEK1
- Gene Name
- MAP2K1
Antibody
- Host Species
- Rabbit
- Clonality
- Monoclonal
- Clone
- 131
- Isotype
- IgG
- Origin Species
- Human
Immunogen
- Immunogen
- Recombinant Mouse MEK1/MAP2K1 Protein
- Immunogen Type
- Recombinant Immunogen
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- 0.2 µm filtered solution in PBS
- Volume
- 100 Microliter
- Preservative
- No
- Purification Method
- Protein A Affinity
Storage & Handling
- Storage Conditions
- Store at 2°C to 8°C for up to 1 month. Aliquot and store at -20°C to -80°C for up to 1 year. Avoid freeze/thaw cycles.
- Recommended Storage Buffer
- PBS