Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-LC3A Polyclonal Antibody. Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-LC3A Polyclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cytoplasm, Autophagosome, Cytoplasmic Vesicle. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) LC3A Antibody (D) Composite.
MAP1LC3A / LC3A Antibody (FITC)
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Immunofluorescence, Western Blot |
| Reactivity: | Human, Mouse, Rat |
| Format: | Liquid |
$483.00each
Catalog Number: LS-C775812-100
Catalog Number: LS-C775812
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- Description
- LC3A antibody LS-C775812 is an FITC-conjugated rabbit polyclonal antibody to LC3A (MAP1LC3A) from human. It is reactive with human, mouse and rat. Validated for IF and WB.
- Application
- IF, WB
- Reactivity
- Hu, Ms, Rt
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- ATG8E | LC3 | LC3A | MAP1BLC3 | MAP1LC3A | MAP1A/1B light chain 3 A | MAP1A/MAP1B LC3 A | MAP1A/MAP1B light chain 3 A | MAP1ALC3
- Recommended Dilution: IF/ICC
- 1:100
- Recommended Dilution: WB
- 1:1000
- UniProt Number
- Q9H492
- NCBI GenBank
- NM_032514 | NP_115903.1
- SwissProt
- Q9H492
Target
- Target
- Human MAP1LC3A / LC3A
- Antigen Species
- Human
- Epitope
- Detects ~14 kDa. Band at 50 kDa is LC3A complex.
- Gene Name
- MAP1LC3A
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Origin Species
- Human
Immunogen
- Immunogen
- Synthetic peptide from the N-Terminus of Human LC3A (aa. 1-12)
- Immunogen Type
- Synthetic Peptide (Linear)
Format
- Conjugate/Label
- Fluorescein Isothiocyanate
- Format
- Liquid
- Formulation
- PBS, 0.09% Sodium Azide, 50% Glycerol
- Concentration
- 1 mg/mL
- Preservative
- Yes
- Purification Method
- Affinity Purified
Storage & Handling
- Storage Conditions
- Store at -20°C.
- Recommended Storage Buffer
- PBS