Flow cytometry of pig peripheral blood monocytes with Mouse anti-Pig Macrophages followed by Goat anti-Mouse IgG:FITC. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Macrophage Antibody (clone BA4D5, FITC)

Primary AntibodyLSBio Guarantee

Antibody:	Mouse Monoclonal
Applications:	Flow Cytometry
Reactivity:	Pig
Format:	Liquid

$540.00each

Catalog Number: LS-C188281-0.1

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Overview

Description: Macrophage antibody LS-C188281 is an FITC-conjugated mouse monoclonal antibody to pig Macrophage. Validated for Flow.
Application: FC
Reactivity: Por
Intended Use: Research Use Only
Guarantees: This antibody carries the LSBio 100% Guarantee. ✓
Category: Antibodies > Primary Antibodies > Monoclonal Antibodies
Alternative Names: Mature Macrophage Marker
Recommended Dilution: FC: 0.0423611111111111

Target

Target: Pig Macrophage
Antigen Species: Pig
Epitope: Recognizes porcine cells of the monocyte/macrophage lineage. Expression of the antigen is increased with maturation, with higher expression on peritoneal and alveolar macrophages. Some expression has also been observed on peripheral blood lymphocytes. The antigen recognized by the antibody has a broad tissue distribution. Clone BA4D5 stains macrophages in a range of tissues, including the thymus, spleen periarteriolar lymphoid sheath (PALS), spleen red pulp and the Peyers patches. It is believed that clone BA4D5 may be specific for porcine CD68, although the protein recognized by this has not yet been fully characterized. The protein is expressed on the cell surface, although it is most abundantly expressed in the cytoplasm.

Antibody

Host Species: Mouse
Clonality: Monoclonal
Clone: BA4D5
Isotype: IgG2b

Immunogen

Immunogen: Porcine alveolar macrophages.
Immunogen Type: Cell-Based Immunization

Format

Conjugate/Label: Fluorescein Isothiocyanate
Format: Liquid
Formulation: PBS, 0.09% Sodium Azide, 1% BSA
Concentration: 0.1 mg/mL
Preservative: Yes
Purification Method: Protein G Affinity

Storage & Handling

Storage Conditions: Store at 4°C or at -20°C. Store undiluted. Avoid freeze-thaw cycles. Protect from light. Microcentrifugation recommended if solution contains precipitate.
Recommended Storage Buffer: PBS