Sheep anti Lactoperoxidase antibody was used to detect Lactoperoxidase under reducing (R) and non-reducing (NR) conditions. Reduced samples of purified target proteins contained 4% BME and were boiled for 5 minutes. Samples of ~1ug of protein per lane were run by SDS-PAGE. Protein was transferred to nitrocellulose and probed with 1:3000 dilution of primary antibody. Detection shown was using Dylight 488 conjugated secondary antibody. Images were collected using the BioRad VersaDoc System
LPO / Lactoperoxidase Antibody (Lyophilized, Biotin)
Primary AntibodyLSBio Guarantee
| Antibody: | Sheep Polyclonal |
| Applications: | Enzyme-Linked Immunosorbent Assay, Immunoprecipitation, Western Blot |
| Reactivity: | Bovine |
| Format: | Lyophilized |
$558.00each
Catalog Number: LS-C745192-100
Catalog Number: LS-C745192
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- Description
- Lactoperoxidase antibody LS-C745192 is a biotin-conjugated sheep polyclonal antibody to bovine Lactoperoxidase (LPO). Validated for ELISA, IP and WB.
- Application
- ELISA, IP, WB
- Reactivity
- Bov
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- Lactoperoxidase | Salivary peroxidase | LPO | SPO | SAPX
- Recommended Dilution: ELISA
- 1:500 - 1:2000
- Recommended Dilution: IP
- 1:100
- Recommended Dilution: WB
- 1:50 - 1:500
- UniProt Number
- P22079
- SwissProt
- P22079
Target
- Target
- Bovine LPO / Lactoperoxidase
- Antigen Species
- Bovine
- Epitope
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Sheep Serum as well as purified and partially purified Lactoperoxidase [Bovine Milk]. Cross reactivity against Lactoperoxidase from other sources is unknown.
- Gene Name
- LPO
Antibody
- Host Species
- Sheep
- Clonality
- Polyclonal
- Isotype
- IgG
- Origin Species
- Human
Immunogen
- Immunogen
- Lactoperoxidase [Bovine Milk]
Format
- Conjugate/Label
- Biotin
- Format
- Lyophilized
- Formulation
- Lyophilized from 10 mg/mL BSA (Protease and Ig free), 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
- Concentration
- 1 mg/mL
- Preservative
- Yes
- Purification Method
- Ion Exchange Chromatography
Storage & Handling
- Storage Conditions
- Store vial at -20°C or below prior to opening. Dilute 1:10 to minimize loss. Store the vial at -20°C or below after dilution. Avoid freeze-thaw cycles.
- Recommended Storage Buffer
- 0.02 M Potassium Phosphate|0.15 M NaCl