Anti-LIM Kinase Antibody - Western Blot. Western blot of Affinity Purified anti-LIM Kinase antibody shows detection of a 73 kD band corresponding to LIM kinase in lysates from mouse brain. Approximately 18 ug of lysate was run on a SDS-PAGE and transferred onto nitrocellulose followed by reaction with a 1:500 dilution of anti-LIM kinase antibody. The doublet band at ~75 kD may represent phosphorylated and non-phosphorylated forms of the protein. The identity of the strong lower molecular weight band at approximately 20 kD is unknown. Signal was detected using standard techniques.
LIMK1 / LIMK Antibody (aa620-647)
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| Antibody: | Rabbit Polyclonal |
| Applications: | Enzyme-Linked Immunosorbent Assay, Immunofluorescence, Immunohistochemistry (general), Western Blot |
| Reactivity: | Human, Non-Human Primate |
| Format: | Liquid |
$579.00each
Catalog Number: LS-C18837-100
Catalog Number: LS-C18837
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- Description
- LIMK antibody LS-C18837 is an unconjugated rabbit polyclonal antibody to LIMK (LIMK1) (aa620-647) from human. It is reactive with human, chimpanzee and orangutan. Validated for ELISA, IF and WB.
- Application
- ELISA, IF, IHC, WB
- Reactivity
- Hu, NHP
- Predicted Reactivity
- Mouse, Non-Human Primate, Rat
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- LIM domain kinase 1 | Lim kinase 1 | LIMK-1 | LIM kinase | LIMK1 | LIMK
- Recommended Dilution: ELISA
- 1:8000 - 1:36000
- Recommended Dilution: WB
- 1:500 - 1:2000
- UniProt Number
- P53667
- NCBI GenBank
- NM_002314 | NP_002305.1
- SwissProt
- P53667
Target
- Target
- Human LIMK1 / LIMK
- Antigen Species
- Human
- Epitope
- A BLAST analysis was used to suggest reactivity with this protein from human, chimpanzee and orangutan based on 100% homology for the immunogen sequence. Expect cross reactivity with LIM kinase from mouse and rat sources as only a single amino acid residue change is found within the immunogen sequence (94% positive by BLAST). Cross reactivity with LIM kinase homologues from other sources has not been determined.
- Gene Name
- LIMK1
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Isotype
- IgG
Immunogen
- Immunogen
- This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to the C-terminal region near aa 620-647 of human LIM kinase protein.
- Immunogen Type
- Synthetic Peptide (Linear)
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
- Concentration
- 1 mg/mL
- Preservative
- Yes
- Purification Method
- Affinity Purified
Storage & Handling
- Storage Conditions
- Store vial at -20°C prior to opening. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20°C or below. Avoid freeze-thaw cycles.
- Recommended Storage Buffer
- 0.02 M Potassium Phosphate|0.15 M NaCl