Western blot analysis of extracts of various cell lines, using LGALS3 antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 1s.
LGALS3 / Galectin 3 Antibody
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Western Blot |
| Reactivity: | Human, Mouse |
| Format: | Liquid |
$293.00each
Catalog Number: LS-C748558-20
Catalog Number: LS-C748558
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- Description
- Galectin 3 antibody LS-C748558 is an unconjugated rabbit polyclonal antibody to Galectin 3 (LGALS3) from human. It is reactive with human and mouse. Validated for WB.
- Application
- WB
- Reactivity
- Hu, Ms
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- 35 kd lectin | 35 kDa lectin | CBP35 | GAL3 | Galactoside-binding protein | GALIG | IgE-binding protein | Laminin-binding protein | Gal-3 | GALBP | MAC-2 | Mac-2 antigen | L31 | MAC2 | CBP 35 | Galactose-specific lectin 3 | Galectin-3 | L-31 | Lectin L-29 | LGALS3
- Recommended Dilution: WB
- 1:500 - 1:2000
- UniProt Number
- P17931
- NCBI GenBank
- NM_002306 | NP_002297.2
- SwissProt
- P17931
Target
- Target
- Human LGALS3 / Galectin 3
- Antigen Species
- Human
- Gene Name
- LGALS3
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Isotype
- IgG
- Origin Species
- Human
Immunogen
- Immunogen
- Recombinant fusion protein containing a sequence corresponding to aa 1-250 of human LGALS3 (NP_002297.2). MADNFSLHDALSGSGNPNPQGWPGAWGNQPAGAGGYPGASYPGAYPGQAPPGAYPGQAPPGAYPGAPGAYPGAPAPGVYPGPPSGPGAYPSSGQPSATGAYPATGPYGAPAGPLIVPYNLPLPGGVVPRMLITILGTVKPNANRIALDFQRGNDVAFHFNPRFNENNRRVIVCNTKLDNNWGREERQSVFPFESGKPFKIQVLVEPDHFKVAVNDAHLLQYNHRVKKLNEISKLGISGDIDLTSASYTMI
- Immunogen Type
- Fusion Protein (e.g., GST, MBP tag)
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- PBS, pH 7.3, 0.02% Sodium Azide, 50% Glycerol
- Volume
- 20 Microliter
- Preservative
- Yes
- Purification Method
- Affinity Purified
Storage & Handling
- Storage Conditions
- Store at -20°C. Avoid freeze-thaw cycles.
- Recommended Storage Buffer
- PBS