LGALS3 / Galectin 3 Antibody (clone M3/38, Biotin)
Primary AntibodyLSBio Guarantee
| Antibody: | Rat Monoclonal |
| Applications: | Enzyme-Linked Immunosorbent Assay, Flow Cytometry, Immunohistochemistry (general), Immunohistochemistry, Frozen, Immunohistochemistry, Paraffin |
| Reactivity: | Mouse |
| Format: | Liquid |
$365.00each
Catalog Number: LS-C62616-100
Catalog Number: LS-C62616
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- Description
- Galectin 3 antibody LS-C62616 is a biotin-conjugated rat monoclonal antibody to mouse Galectin 3 (LGALS3). Validated for ELISA, Flow and IHC.
- Application
- ELISA, FC, IHC, IHC-F, IHC-P
- Reactivity
- Ms
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Monoclonal Antibodies
- Alternative Names
- 35 kd lectin | 35 kDa lectin | CBP35 | GAL3 | Galactoside-binding protein | GALIG | IgE-binding protein | Laminin-binding protein | Gal-3 | GALBP | MAC-2 | Mac-2 antigen | L31 | MAC2 | CBP 35 | Galactose-specific lectin 3 | Galectin-3 | L-31 | Lectin L-29 | LGALS3
- Recommended Dilution: FC
- 0.5 µg/10E6 cells
- Recommended Dilution: IHC-P
- 1:1000 - 1:2000
- UniProt Number
- P17931
- NCBI GenBank
- NM_002306 | NP_002297.2
- SwissProt
- P17931
Target
- Target
- Mouse LGALS3 / Galectin 3
- Antigen Species
- Mouse
- Epitope
- The antibody recognizes a 32,000 dalton surface antigen found on a subpopulation of mouse macrophages. It reactswith peritoneal exudate macrophages where the exudate is provoked by thioglycollate, protease peptone (20%),macrophages of lymphoid and non-lymphoid tissues, interdigitating dendritic cells and Langerhans cells. Mac-2 isalso expressed in the cytoplasm on non-elicited resident macrophages; 5% are strongly reactive, the remaining 95%show much weaker staining.The antibody does not react with peritoneal exudate macrophages where the exudate is provoked by Listeriamonocytogenes, lipopolysaccharide or concanavalin A. It also does not react with peritoneal macrophages, splenicmacrophages, granulocytes, thymocytes, peripheral lymph node cells and with 99% of bone marrow cells.
- Gene Name
- LGALS3
Antibody
- Host Species
- Rat
- Clonality
- Monoclonal
- Clone
- M3/38
- Isotype
- IgG2a
Immunogen
- Immunogen
- Plasma membrane glycoproteins from C57BL/6 mouse thioglycollate-elicited peritoneal exudate.
- Immunogen Type
- Tissue Extract
Format
- Conjugate/Label
- Biotin
- Format
- Liquid
- Formulation
- PBS, 0.02% Sodium Azide, BSA
- Concentration
- 0.1 mg/mL
- Preservative
- Yes
- Purification Method
- Protein G Affinity
Storage & Handling
- Storage Conditions
- Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
- Recommended Storage Buffer
- PBS