Western blot analysis of extracts of various cell lines, using LGALS1 antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 30s.
LGALS1 / Galectin 1 Antibody
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Immunohistochemistry (general), Western Blot |
| Reactivity: | Human, Mouse, Rat |
| Format: | Liquid |
$281.00each
Catalog Number: LS-C497920-20
Catalog Number: LS-C497920
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- Description
- Galectin 1 antibody LS-C497920 is an unconjugated rabbit polyclonal antibody to Galectin 1 (LGALS1) from human. It is reactive with human, mouse and rat. Validated for IHC and WB.
- Application
- IHC, WB
- Reactivity
- Hu, Ms, Rt
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- 14 kDa lectin | 14 kDa laminin-binding protein | 14 kd lectin | GAL1 | Galectin 1 | Galectin-1 | HBL | Lactose-binding lectin 1 | LGALS1 | Galaptin | GBP | HLBP14 | HPL | S-Lac lectin 1 | Gal-1
- Recommended Dilution: IHC
- 1:50 - 1:200
- Recommended Dilution: WB
- 1:1000 - 1:4000
- UniProt Number
- P09382
- NCBI GenBank
- NM_002305 | NP_002296.1
- SwissProt
- P09382
Target
- Target
- Human LGALS1 / Galectin 1
- Antigen Species
- Human
- Gene Name
- LGALS1
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Isotype
- IgG
Immunogen
- Immunogen
- Recombinant fusion protein containing a sequence corresponding to amino acids 1-135 of human LGALS1 (NP_002296.1). MACGLVASNLNLKPGECLRVRGEVAPDAKSFVLNLGKDSNNLCLHFNPRFNAHGDANTIVCNSKDGGAWGTEQREAVFPFQPGSVAEVCITFDQANLTVKLPDGYEFKFPNRLNLEAINYMAADGDFKIKCVAFD
- Immunogen Type
- Fusion Protein (e.g., GST, MBP tag)
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- PBS, pH 7.3, 0.02% Sodium Azide, 50% Glycerol
- Volume
- 20 Microliter
- Preservative
- Yes
- Purification Method
- Affinity Purified
Storage & Handling
- Storage Conditions
- Store at -20°C. Avoid freeze-thaw cycles.
- Recommended Storage Buffer
- PBS