Anti-LDB1 Antibody - Western Blot. Western blot of affinity purified anti-LDB1 antibody shows detection of LDB1 protein (arrowhead) in Jurkat whole cell lysate. Approximately 30 ug of lysate was loaded prior to separation and transfer to nitrocellulose. Primary antibody was used at a 1:1800 dilution in 5% BLOTTO in PBS reacted overnight at 4C. The membrane was washed and reacted with a 1:20000 dilution of DyLight800 conjugated Gt-a-Rabbit IgG [H&L] MX ( for 45 min at room temperature (800 nm channel, green). Molecular weight estimation was made by comparison to prestained MW markers in lane M (700 nm channel, red). Fluorescence image was captured using the Odyssey Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.