Western blot analysis of extracts of U937 cells, using Phospho-LCP2-Y145 antibody at 1:1000 dilution. U-937 cells were treated by PMA/TPA (200nM) for 30 minutes. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. Blocking buffer: 3% BSA.An ECL Kit was used for detection and the exposure time was 10s.
LCP2 / SLP-76 Antibody (phospho-Tyr145)
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Western Blot |
| Reactivity: | Human, Mouse |
| Format: | Liquid |
$293.00each
Catalog Number: LS-C750622-20
Catalog Number: LS-C750622
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- Description
- SLP-76 antibody LS-C750622 is an unconjugated rabbit polyclonal antibody to SLP-76 (LCP2) (pTyr145) from human. It is reactive with human and mouse. Validated for WB.
- Application
- WB
- Reactivity
- Hu, Ms
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- 76 kDa tyrosine phosphoprotein | Lymphocyte cytosolic protein 2 | LCP2 | SLP-76 | SLP-76 tyrosine phosphoprotein | SLP76
- Recommended Dilution: WB
- 1:500 - 1:2000
- UniProt Number
- Q13094
- NCBI GenBank
- NM_005565 | NP_005556.1
- SwissProt
- Q13094
Target
- Target
- Human LCP2 / SLP-76
- Antigen Species
- Human
- Gene Name
- LCP2
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Isotype
- IgG
- Origin Species
- Human
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- PBS, pH 7.3, 0.02% Sodium Azide, 50% Glycerol
- Volume
- 20 Microliter
- Preservative
- Yes
- Purification Method
- Affinity Purified
Storage & Handling
- Storage Conditions
- Store at -20°C. Avoid freeze-thaw cycles.
- Recommended Storage Buffer
- PBS