LCP2 / SLP-76 Antibody (aa216-434, clone SLP-76/03, Concentrated)
Primary AntibodyLSBio Guarantee
| Antibody: | Mouse Monoclonal |
| Applications: | Western Blot |
| Reactivity: | Human |
| Format: | Liquid |
$556.00each
Catalog Number: LS-C204152-0.1
Catalog Number: LS-C204152
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- Description
- SLP-76 antibody LS-C204152 is an unconjugated mouse monoclonal antibody to human SLP-76 (LCP2) (aa216-434). Validated for WB.
- Application
- WB
- Reactivity
- Hu
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Monoclonal Antibodies
- Alternative Names
- 76 kDa tyrosine phosphoprotein | Lymphocyte cytosolic protein 2 | LCP2 | SLP-76 | SLP-76 tyrosine phosphoprotein | SLP76
- UniProt Number
- Q13094
- NCBI GenBank
- NM_005565 | NP_005556.1
- SwissProt
- Q13094
Target
- Target
- Human LCP2 / SLP-76
- Antigen Species
- Human
- Epitope
- SLP-76 (SH2 domain-containing leukocyte protein) is an adaptor molecule that is involved in T cell development and activation. Thymocytes from transgenic mice expressing a SLP-76 mutant with deletion of the amino terminus are unable to develop to CD4-CD8- stage. Mature T cells do develop in mice expressing SLP-76 with mutations in proline rich region or SH2 domain, but they fail to upregulate CD69 or proliferate appropriately in response to anti-CD3 stimulation. SLP-76 is cytosolic protein identified as phosphoprotein migrating at 76 kD in SDS-PAGE. Amino-terminal domain of SLP-76 containing several tyrosines is followed by PR (proline rich) region and an SH2 domain. SLP-76 associates with LAT via Gads or Grb 2. The PR region of SLP-76 constitutively associates with carboxy-terminal SH3 domain of Gads and P-1 segment of the PR region was also identified that associates with the SH3 domain of PLC-gamma1. The PR region therefore provides another pathway for PLC-gamma1 recruitment to the LAT signalling complex in T cells. The SH2 domain of SLP-76 has been identified as the region involved in binding the serine/threonine kinase HPK1. HPK1 may act as both a positive and a negative regulator by promoting the Jnk mitogen activated protein kinase (MAPK) pathway and inhibiting the pathway leading to AP-1 activation.
- Gene Name
- LCP2
Antibody
- Host Species
- Mouse
- Clonality
- Monoclonal
- Clone
- SLP-76/03
- Isotype
- IgG2b
Immunogen
- Immunogen
- A fusion protein representing amino acids 216-434 of human SLP-76
- Immunogen Type
- Fusion Protein (e.g., GST, MBP tag)
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- PBS, pH 7.4, 15 mM Sodium Azide
- Concentration
- 1 mg/mL
- Preservative
- Yes
- Purification Method
- Other
Storage & Handling
- Storage Conditions
- Store at 2-8°C.
- Recommended Storage Buffer
- PBS