Detection of endogenous human LAG-3 by FACS analysis using anti-LAG-3 (human), mAb (17B4) (ATTO 488) . . Human PBMC were stimulated (B) or not (A) with 1 ug/ml of superantigen SEB. After 2 days, PBMC were stained with 10 ug/ml (1 ug/0.5x106 cells) of anti-LAG-3 (human), mAb (17B4) (ATTO 488) and analyzed by flow cytometry. This image was taken for the unconjugated form of this product. Other forms have not been tested.

LAG3 Antibody (aa70-99, clone 17B4, Atto 488)

Primary AntibodyLSBio Guarantee

Antibody:	Mouse Monoclonal
Applications:	Flow Cytometry, Functional Assay, Immunocytochemistry, Immunohistochemistry (general), Immunohistochemistry, Frozen, Immunoprecipitation, Western Blot
Reactivity:	Human
Format:	Liquid

$508.00each

Catalog Number: LS-C344747-100

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Overview

Description: LAG3 antibody LS-C344747 is an Atto 488-conjugated mouse monoclonal antibody to human LAG3 (aa70-99). Validated for Flow, Func, ICC, IHC, IP and WB.
Application: FC, Func, ICC, IHC, IHC-F, IP, WB
Reactivity: Hu
Intended Use: Research Use Only
Guarantees: This antibody carries the LSBio 100% Guarantee. ✓
Category: Antibodies > Primary Antibodies > Monoclonal Antibodies
Alternative Names: CD223 antigen | LAG-3 | Protein FDC | CD223 | FDC | LAG3 | Lymphocyte-activation gene 3
Recommended Dilution: FC: 1:100
UniProt Number: P18627
NCBI GenBank: NM_002286
SwissProt: P18627

Target

Target: Human LAG3
Antigen Species: Human
Epitope: Recognizes the 30 aa extra-loop of the first N-terminal D1 domain of human LAG-3.
Gene Name: LAG3

Antibody

Host Species: Mouse
Clonality: Monoclonal
Clone: 17B4
Isotype: IgG1

Immunogen

Immunogen: Synthetic peptide corresponding to 30 aa (GPPAAAPGHPLAPGPHPAAPSSWGPRPRRY) from the first N-Terminus D1 domain of human LAG-3 (lymphocyte activation gene-3). Percent identity by BLAST analysis: Human (100%); Gorilla (93%); Gibbon (90%); Monkey (83%).
Immunogen Type: Synthetic Peptide (Linear)