Anti-Lac I Antibody - Western Blot. Western blot of affinity purified anti-Lac I antibody shows detection of a 38 kD band corresponding to recombinant Lac I (arrowhead). The blot was blocked with 5% BLOTTO in TTBS overnight at 4C. Primary antibody was used at a 1:1000 dilution followed by reaction with a 1:10000 dilution of HRP goat anti-rabbit IgG. ECL was used for detection. Personal communication, S. Hughes & M. Abram, NCI, Bethesda, MD.
LacI / Lac Repressor Antibody
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general), Western Blot |
| Reactivity: | Bacteria, E. coli |
| Format: | Liquid |
$579.00each
Catalog Number: LS-C60143-100
Catalog Number: LS-C60143
For bulk orders or custom formulations:
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- Description
- Lac Repressor antibody LS-C60143 is an unconjugated rabbit polyclonal antibody to Lac Repressor (LacI) from e. coli. It is reactive with e. coli and s. sonnei. Validated for ELISA and WB.
- Application
- ELISA, IHC, WB
- Reactivity
- Bac, E.col
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Recommended Dilution: ELISA
- 1:90000 - 1:100000
- Recommended Dilution: WB
- 1:500 - 1:2000
Target
- Target
- E. coli LacI / Lac Repressor
- Antigen Species
- E. coli
- Epitope
- This Anti-Lac I product was affinity purified from monospecific antiserum by immunoaffinity chromatography. This antibody reacts with recombinant Lac I protein. A BLAST analysis was used to suggest cross-reactivity with Lac I from DNA-binding transcriptional repressor in Escherichia coli (strain DH10B) and transcriptional repressor of the lac operon in Shigella sonnei (strain Ss046) based on a 100% homology with the immunizing sequence. Cross-reactivity with Lac I from other sources has not been determined.
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Isotype
- IgG
Immunogen
- Immunogen
- This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a full-length recombinant Lac I protein.
- Immunogen Type
- Recombinant Immunogen
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
- Concentration
- 1 mg/mL
- Preservative
- Yes
- Purification Method
- Affinity Purified
Storage & Handling
- Storage Conditions
- Short term: 4°C Long term: Aliquot and store at -20°C. Avoid freeze-thaw cycles.
- Recommended Storage Buffer
- 0.02 M Potassium Phosphate|0.15 M NaCl