IHC testing of human colon carcinoma with Cytokeratin 20 antibody (clone KRT20/1991). Required HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min followed by cooling at RT for 20 min.
KRT20 / CK20 / Cytokeratin 20 Antibody (clone KRT20/1991)
Primary AntibodyLSBio Guarantee
| Antibody: | Mouse Monoclonal |
| Applications: | Immunohistochemistry, Paraffin, Western Blot |
| Reactivity: | Human |
| Format: | Liquid |
$379.00each
Catalog Number: LS-C782719-20
Catalog Number: LS-C782719
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- Description
- Cytokeratin 20 antibody LS-C782719 is an unconjugated mouse monoclonal antibody to human Cytokeratin 20 (KRT20 / CK20). Validated for IHC and WB.
- Application
- IHC-P, WB
- Reactivity
- Hu
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Monoclonal Antibodies
- Alternative Names
- Cytokeratin-20 | Cytokeratin 20 | CK20 | KRT20 | K20 | Keratin 20 | Keratin-20 | KRT21 | CK-20 | Protein IT
- Recommended Dilution: IHC-P
- 1 - 2 µg/ml
- Recommended Dilution: WB
- 0.5 - 2 µg/ml
- UniProt Number
- P35900
- NCBI GenBank
- CAA51914.1 | X73502
- SwissProt
- P35900
Target
- Target
- Human KRT20 / CK20 / Cytokeratin 20
- Antigen Species
- Human
- Epitope
- This antibody has been extensively validated, including cross-reactivity testing against 19,000 full-length proteins by HuProt(TM) microarray analysis.
- Gene Name
- KRT20
Antibody
- Host Species
- Mouse
- Clonality
- Monoclonal
- Clone
- KRT20/1991
- Isotype
- IgG1
- Light Chain
- Kappa Light Chain
- Origin Species
- Human
Immunogen
- Immunogen
- A portion of amino acids 196-323 from the human protein was used as the immunogen for this Cytokeratin 20 antibody.
- Immunogen Type
- Peptide
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- 1X PBS, 0.1 mg/ml BSA, 0.05% sodium azide
- Concentration
- 0.2 mg/mL
- Preservative
- Yes
- Purification Method
- Protein G Affinity
Storage & Handling
- Storage Conditions
- Store at 2-8°C.
- Recommended Storage Buffer
- 1X PBS