Western blot analysis of extracts of various cell lines, using KRT2 antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 90s.
KRT2 / CK2 / Cytokeratin 2 Antibody
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Western Blot |
| Reactivity: | Human, Mouse, Rat |
| Format: | Liquid |
$281.00each
Catalog Number: LS-C497083-20
Catalog Number: LS-C497083
For bulk orders or custom formulations:
Request a QuoteOverview
- Description
- Cytokeratin 2 antibody LS-C497083 is an unconjugated rabbit polyclonal antibody to Cytokeratin 2 (KRT2 / CK2) from human. It is reactive with human, mouse and rat. Validated for WB.
- Application
- WB
- Reactivity
- Hu, Ms, Rt
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- Cytokeratin-2e | CK-2e | Epithelial keratin-2e | K2e | Keratin 2 | Keratin-2e | Krt2e | KRT2 | KRT2A | KRTE | Keratin-2 epidermis | Type-II keratin Kb2
- Recommended Dilution: WB
- 1:500 - 1:2000
- UniProt Number
- P35908
- NCBI GenBank
- NM_000423 | NP_000414.2
- SwissProt
- P35908
Target
- Target
- Human KRT2 / CK2 / Cytokeratin 2
- Antigen Species
- Human
- Gene Name
- KRT2
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Isotype
- IgG
Immunogen
- Immunogen
- Recombinant fusion protein containing a sequence corresponding to amino acids 1-220 of human KRT2 (NP_000414.2). MSCQISCKSRGRGGGGGGFRGFSSGSAVVSGGSRRSTSSFSCLSRHGGGGGGFGGGGFGSRSLVGLGGTKSISISVAGGGGGFGAAGGFGGRGGGFGGGSSFGGGSGFSGGGFGGGGFGGGRFGGFGGPGGVGGLGGPGGFGPGGYPGGIHEVSVNQSLLQPLNVKVDPEIQNVKAQEREQIKTLNNKFASFIDKVRFLEQQNQVLQTKWELLQQMNVGT
- Immunogen Type
- Fusion Protein (e.g., GST, MBP tag)
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- PBS, pH 7.3, 0.02% Sodium Azide, 50% Glycerol
- Volume
- 20 Microliter
- Preservative
- Yes
- Purification Method
- Affinity Purified
Storage & Handling
- Storage Conditions
- Store at -20°C. Avoid freeze-thaw cycles.
- Recommended Storage Buffer
- PBS