Immunofluorescence staining of MCF-7 cells at a dilution of 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4 °C.The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L) .
KRT1 / CK1 / Cytokeratin 1 Antibody
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Enzyme-Linked Immunosorbent Assay, Immunofluorescence, Immunohistochemistry (general), Immunohistochemistry, Paraffin |
| Reactivity: | Human |
| Format: | Liquid |
$300.00each
Catalog Number: LS-C673185-50
Catalog Number: LS-C673185
For bulk orders or custom formulations:
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- Description
- Cytokeratin 1 antibody LS-C673185 is an unconjugated rabbit polyclonal antibody to human Cytokeratin 1 (KRT1 / CK1). Validated for ELISA, IF and IHC.
- Application
- ELISA, IF, IHC, IHC-P
- Reactivity
- Hu
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- 67 kDa cytokeratin | CK-1 | CK1 | Cytokeratin 1 | Cytokeratin-1 | Epidermolytic hyperkeratosis 1 | EHK | EHK1 | Hair alpha protein | EPPK | KRT1 | NEPPK | K1 | Keratin-1 | KRTA | Type-II keratin Kb1 | Type II keratin | Keratin 1 | KRT1A
- UniProt Number
- P04264
- NCBI GenBank
- NM_006121 | NP_006112.3
- SwissProt
- P04264
Target
- Target
- Human KRT1 / CK1 / Cytokeratin 1
- Antigen Species
- Human
- Gene Name
- KRT1
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Isotype
- IgG
Immunogen
- Immunogen
- Recombinant Human Keratin, type II cytoskeletal 1 protein (267-438AA)
- Immunogen Type
- Recombinant Immunogen
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- 0.01 M PBS, pH 7.4, 0.03% ProClin™ 300, 50% Glycerol
- Concentration
- 4.89 mg/mL
- Preservative
- Yes
- Purification Method
- Protein G Affinity
Storage & Handling
- Storage Conditions
- Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
- Recommended Storage Buffer
- 0.01 M PBS