LSBio
KLF4 Antibody

IHC analysis of KLF4 using anti-KLF4 antibody. KLF4 was detected in paraffin-embedded section of rat small intestine tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2?g/ml rabbit anti-KLF4 Antibody overnight at 4?C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37?C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.

KLF4 Antibody

Primary AntibodyLSBio Guarantee
Antibody:Rabbit Polyclonal
Applications:Immunohistochemistry, Paraffin, Western Blot
Reactivity:Human, Mouse, Rat
Format:Lyophilized
See all Human KLF4 antibodies →
$480.00each
Catalog Number: LS-C756373-100

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Overview

Description
KLF4 antibody LS-C756373 is an unconjugated rabbit polyclonal antibody to KLF4 from human. It is reactive with human, mouse and rat. Validated for IHC and WB.
Application
IHC-P, WB
Reactivity
Hu, Ms, Rt
Intended Use
Research Use Only
Guarantees
This antibody carries the LSBio 100% Guarantee. ✓
Category
Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names
EZF | GKLF | Kruppel-like factor 4 (gut) | Hezf | KLF4 | Krueppel-like factor 4
UniProt Number
O43474
SwissProt
O43474
Recommended Dilution
ApplicationDilution
IHC-P0.5 - 1 µg/ml
WB0.1 - 0.5 µg/ml

Target

Target
Human KLF4
Antigen Species
Human
Epitope
No cross reactivity with other proteins.
Gene Name
KLF4

Antibody

Host Species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Origin Species
Human

Immunogen

Immunogen
A synthetic peptide corresponding to a sequence of human KLF4 (EKTLRQAGAPNNRWREELSHMKRLPPVLPGRPYDLA).
Immunogen Type
Synthetic Peptide (Linear)

Format

Conjugate/Label
Unconjugated
Format
Lyophilized
Formulation
Lyophilized from 0.2mg Na2HPO4, 0.9mg NaCl, 0.05mg sodium azide, 4mg Trehalose
Preservative
Yes
Purification Method
Affinity Purified

Storage & Handling

Storage Conditions
After reconstitution, may be stored at 4°C for 1 month. For long-term storage and to avoid freeze-thaw cycles, aliquot and store at -20°C.
Recommended Storage Buffer
NaCl

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.