Anti-Klotho antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.
KL / Klotho Antibody (aa251-300)
| Antibody: | Rabbit Polyclonal |
| Applications: | Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general), Immunohistochemistry, Paraffin, Western Blot |
| Reactivity: | Human |
| Format: | Liquid |
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- Description
- Klotho antibody LS-B6625 is an unconjugated rabbit polyclonal antibody to human Klotho (KL) (aa251-300). Validated for IHC and WB. Cited in 3 publications.
- Application
- ELISA, IHC, IHC-P, WB
- Reactivity
- Hu
- Intended Use
- Research Use Only
- Guarantees
- This product carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- KL | Klotho
- Recommended Dilution: IHC-P
- 5 µg/ml
- Recommended Dilution: WB
- 0.2 - 1 µg/ml
- UniProt Number
- Q9UEF7
- NCBI GenBank
- NM_004795 | NP_004786.2
- SwissProt
- Q9UEF7
Target
- Target
- KL / Klotho
- Antigen Species
- Human
- Specificity
- aa251-300 of dog, guinea pig, horse, human, mouse, rabbit, rat KL / Klotho
- Epitope
- Human KL / Klotho
- Gene Name
- KL
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Origin Species
- Human
Immunogen
- Immunogen
- Synthetic peptide located between aa251-300 of human KL (Q9UEF7-2). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Monkey, Galago, Marmoset, Mouse, Rat, Elephant, Dog, Bat, Rabbit, Horse, Guinea pig, Platypus (100%); Gibbon, Bovine, Pig, Opossum (92%).
- Immunogen Type
- Synthetic Peptide (Linear)
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- PBS, 0.09% sodium azide, 2% sucrose
- Concentration
- 0.5 mg/mL
- Volume
- 50 Microliter
- Animal Free
- No
- Carrier-Free
- Yes
- Preservative
- Yes
- Purification Method
- Affinity Purified
Storage & Handling
- Storage Conditions
- Short term: Store at 2-8°C. Long term: Aliquot and store at -20°C. Avoid freeze/thaw cycles.
- Recommended Storage Buffer
- PBS
Soluble klotho as a marker of renal fibrosis and podocyte injuries in human kidneys. Cho NJ, Han DJ, Lee JH, Jang SH, Kang JS, Gil HW, Park S, Lee EY. PloS one. 2018 March;13:e0194617.
PubMed: 29590173 · PMC: PMC5874023
Protective effects of klotho on palmitate-induced podocyte injury in diabetic nephropathy. Jeong Suk Kang , Seung Seob Son, Ji-Hye Lee, Seong Woo Lee , Ah Reum Jeong, Eun Soo Lee, Seung-Kuy Cha, Choon Hee Chung, Eun Young Lee. PloS one. 2021 Apr;16:e0250666.
PubMed: 33891667 · PMC: PMC8064606
Down-expression of klotho in canine mammary gland tumors and its prognostic significance. Heaji Chung, Sungin Lee, Geon A Kim, Wan Hee Kim. PloS one. 2022 June;17:e0265248.
PubMed: 35666743 · PMC: PMC9170104