Western Blot of rabbit anti-Eg-5 Antibody. Lane 1: E.coli cell lysate expressing histidine tagged protein. Lane 2: Mouse brain lysate. Lane 3: Recombinant truncated Eg-5. Lane 4: MW. Load: 35 µg per lane for cell lysate. 50 ng of recombinant protein. Primary antibody: Eg-5 antibody at 1:1000 for overnight at 4°C. Secondary antibody: HRP rabbit secondary antibody at 1:40,000 for 60 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: 120 kDa and 72 kDa for Eg5.
KIF11 / EG5 Antibody
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general), Western Blot |
| Reactivity: | Human |
| Format: | Liquid |
$311.00each
Catalog Number: LS-C744615-25
Catalog Number: LS-C744615
For bulk orders or custom formulations:
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- Description
- EG5 antibody LS-C744615 is an unconjugated rabbit polyclonal antibody to human EG5 (KIF11). Validated for ELISA, IHC and WB.
- Application
- ELISA, IHC, WB
- Reactivity
- Hu
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- EG5 | Kinesin family member 11 | Kinesin-like protein 1 | Kinesin-like 1 | Kinesin-like protein KIF11 | MCLMR | TRIP5 | HKSP | KIF11 | KNSL1 | TR-interacting protein 5 | TRIP-5
- Recommended Dilution: ELISA
- 1:10000
- Recommended Dilution: WB
- 1:1000
- UniProt Number
- P52732
- NCBI GenBank
- NM_004523 | NP_004514.2
- SwissProt
- P52732
Target
- Target
- Human KIF11 / EG5
- Antigen Species
- Human
- Epitope
- A BLAST analysis was used to suggest reactivity with human. Cross-reactivity with Eg5 from other sources have not been determined.
- Gene Name
- KIF11
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Origin Species
- Human
Immunogen
- Immunogen
- Eg5 was prepared from whole rabbit serum produced by repeated immunizations with a truncated Eg5 construct expressed in E. coli corresponding to human Eg5 protein.
- Immunogen Type
- Recombinant Immunogen
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
- Concentration
- 46 mg/mL
- Volume
- 25 Microliter
- Preservative
- Yes
- Purification Method
- Other
Storage & Handling
- Storage Conditions
- Store vial at -20°C prior to opening. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20°C or below. Avoid freeze-thaw cycles.
- Recommended Storage Buffer
- 0.02 M Potassium Phosphate|0.15 M NaCl