Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-Rubicon Polyclonal Antibody. Tissue: Colon carcinoma cell line (RKO). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-Rubicon Polyclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Late Endosome, Lysosome, Early Endosome. Magnification: 60X. (A) DAPI nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) Rubicon Antibody. (D) Composite.
KIAA0226 / RUBICON Antibody (FITC)
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Immunofluorescence, Immunohistochemistry (general), Western Blot |
| Reactivity: | Human |
| Format: | Liquid |
$483.00each
Catalog Number: LS-C774369-100
Catalog Number: LS-C774369
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- Description
- RUBICON antibody LS-C774369 is an FITC-conjugated rabbit polyclonal antibody to human RUBICON (KIAA0226). Validated for IF, IHC and WB.
- Application
- IF, IHC, WB
- Reactivity
- Hu
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- Baron | KIAA0226 | RUBICON | Rundataxin
- Recommended Dilution: IF/ICC
- 1:100
- Recommended Dilution: IHC
- 1:50
- Recommended Dilution: WB
- 1:1000
- UniProt Number
- Q92622
- NCBI GenBank
- BAA13215.3
- SwissProt
- Q92622
Target
- Target
- Human KIAA0226 / RUBICON
- Antigen Species
- Human
- Epitope
- Detects ~95 kDa.
- Gene Name
- RUBCN
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Origin Species
- Human
Immunogen
- Immunogen
- Synthetic peptide from the N-Terminus of Human Rubicon
- Immunogen Type
- Synthetic Peptide (Linear)
Format
- Conjugate/Label
- Fluorescein Isothiocyanate
- Format
- Liquid
- Formulation
- PBS, 0.09% Sodium Azide, 50% Glycerol
- Concentration
- 1 mg/mL
- Preservative
- Yes
- Purification Method
- Affinity Purified
Storage & Handling
- Storage Conditions
- Store at -20°C.
- Recommended Storage Buffer
- PBS