Western blot analysis of extracts from HepG2 cells, treated with Na3VO4 (0.3nM, 40mins), using VEGFR2 (Phospho-Tyr1214) antibody.
KDR / VEGFR2 / FLK1 Antibody (phospho-Tyr1214)
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general), Western Blot |
| Reactivity: | Human, Mouse, Rat |
| Format: | Liquid |
$365.00each
Catalog Number: LS-C825284-100
Catalog Number: LS-C825284
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- Description
- FLK1 antibody LS-C825284 is an unconjugated rabbit polyclonal antibody to FLK1 (KDR / VEGFR2) (pTyr1214) from human. It is reactive with human, mouse and rat. Validated for ELISA, IHC and WB.
- Application
- ELISA, IHC, WB
- Reactivity
- Hu, Ms, Rt
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- CD309 | Fetal liver kinase 1 | Fetal liver kinase-1 | KDR | FLK-1 | FLK1 | Kinase insert domain receptor | Quek1 | VEGFR | VEGFR2 | Soluble VEGFR2 | VEGF receptor 2 | VEGFR-2 | CD309 antigen | VEGF-R2
- Recommended Dilution: ELISA
- 1:10000
- Recommended Dilution: IHC
- 1:50 - 1:100
- Recommended Dilution: WB
- 1:500 - 1:1000
- UniProt Number
- P35968
- NCBI GenBank
- NM_002253 | NP_002244.1
- SwissProt
- P35968
Target
- Target
- Human KDR / VEGFR2 / FLK1
- Antigen Species
- Human
- Epitope
- VEGFR2 (Phospho-Tyr1214) antibody detects endogenous levels of VEGFR2 only when phosphorylated at tyrosine 1214. (Human: Tyr1214; Mouse: Tyr1212; Rat: Tyr1210)
- Gene Name
- KDR
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Isotype
- IgG
- Origin Species
- Human
Immunogen
- Immunogen
- Synthesized phosphopeptide derived from human VEGFR2 around the phosphorylation site of tyrosine 1214 (F-H-YP-D-N).
- Immunogen Type
- Synthetic Peptide (Linear)
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- PBS (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
- Preservative
- Yes
- Purification Method
- Affinity Purified
Storage & Handling
- Storage Conditions
- Store at -20°C for up to 1 year.
- Recommended Storage Buffer
- PBS|150mM NaCl