Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Kir6.1 Monoclonal Antibody, Clone S366-60. Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Kir6.1 Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Membrane. Magnification: 60X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) Kir6.1 Antibody. (D) Composite.
KCNJ8 / Kir6.1 Antibody (clone S366-60, Atto 594)
Primary AntibodyLSBio Guarantee
| Antibody: | Mouse Monoclonal |
| Applications: | Immunofluorescence, Immunohistochemistry (general), Western Blot |
| Reactivity: | Human, Mouse, Rat |
| Format: | Liquid |
$489.00each
Catalog Number: LS-C773817-100
Catalog Number: LS-C773817
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- Description
- Kir6.1 antibody LS-C773817 is an Atto 594-conjugated mouse monoclonal antibody to Kir6.1 (KCNJ8) from human. It is reactive with human, mouse and rat. Validated for IF, IHC and WB.
- Application
- IF, IHC, WB
- Reactivity
- Hu, Ms, Rt
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Monoclonal Antibodies
- Alternative Names
- KCNJ8 | UKATP-1 | KIR6.1
- Recommended Dilution: IF/ICC
- 1:100
- Recommended Dilution: WB
- 1:1000
- UniProt Number
- Q15842
- NCBI GenBank
- NM_004982 | NP_004973.1
- SwissProt
- Q15842
Target
- Target
- Human KCNJ8 / Kir6.1
- Antigen Species
- Human
- Epitope
- Detects ~45 kDa.
- Gene Name
- KCNJ8
Antibody
- Host Species
- Mouse
- Clonality
- Monoclonal
- Clone
- S366-60
- Isotype
- IgG2a
- Origin Species
- Human
Immunogen
- Immunogen
- Fusion protein amino acids 306-424 (Cytoplasmic C-Terminus) of rat Kir6.1
- Immunogen Type
- Fusion Protein (e.g., GST, MBP tag)
Format
- Conjugate/Label
- Atto 594
- Format
- Liquid
- Formulation
- PBS, pH 7.4, 0.1% Sodium Azide, 50% Glycerol
- Concentration
- 1 mg/mL
- Preservative
- Yes
- Purification Method
- Protein G Affinity
Storage & Handling
- Storage Conditions
- Store at -20°C.
- Recommended Storage Buffer
- PBS