LSBio
KARS Antibody (Preservative Free)

Immunofluorescence staining of KARS in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human KARS polyclonal antibody (dilution ratio 1:1000) at 4°C overnight. Then cells were stained with the Alexa Fluor 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm.

KARS Antibody (Preservative Free)

Primary AntibodyLSBio Guarantee
Antibody:Rabbit Polyclonal
Applications:Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Paraffin, Western Blot
Reactivity:Human
Format:Liquid
See all Human KARS antibodies →
$309.00each
Catalog Number: LS-C815641-100

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Overview

Description
KARS antibody LS-C815641 is an unconjugated rabbit polyclonal antibody to human KARS. Validated for ICC, IF, IHC and WB.
Application
ICC, IF, IHC-P, WB
Reactivity
Hu
Intended Use
Research Use Only
Guarantees
This antibody carries the LSBio 100% Guarantee.
Category
Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names
CMTRIB | KARS1 | KARS2 | KRS | Lysine--tRNA ligase | LysRS | KARS | KIAA0070 | Lysine tRNA ligase | Lysyl-tRNA synthetase
Recommended Dilution: IF/ICC
1:300 - 1:10000
Recommended Dilution: IHC-P
1:500 - 1:2000
Recommended Dilution: WB
1:500 - 1:2000
UniProt Number
Q15046
SwissProt
Q15046

Target

Target
Human KARS
Antigen Species
Human
Epitope
Human LysRS/Lysyl tRNA synthetase
Gene Name
KARS1

Antibody

Host Species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Origin Species
Human

Immunogen

Immunogen
A synthetic peptide corresponding to the N-Terminus of Human LysRS/Lysyl tRNA synthetase/KARS
Immunogen Type
Synthetic Peptide (Linear)

Format

Conjugate/Label
Unconjugated
Format
Liquid
Formulation
0.2 µm filtered solution in PBS
Volume
100 Microliter
Preservative
No
Purification Method
Protein A Affinity

Storage & Handling

Storage Conditions
Store at 2°C to 8°C for up to 1 month. Aliquot and store at -20°C to -80°C for up to 1 year. Avoid freeze/thaw cycles.
Recommended Storage Buffer
PBS

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