Western blot analysis of extracts of various cell lines, using ITM2A antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 30s.

ITM2A Antibody

Primary AntibodyLSBio Guarantee

Antibody:	Rabbit Polyclonal
Applications:	Western Blot
Reactivity:	Mouse, Rat
Format:	Liquid

$281.00each

Catalog Number: LS-C497092-20

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Overview

Description: ITM2A antibody LS-C497092 is an unconjugated rabbit polyclonal antibody to ITM2A from human. It is reactive with mouse and rat. Validated for WB.
Application: WB
Reactivity: Ms, Rt
Intended Use: Research Use Only
Guarantees: This antibody carries the LSBio 100% Guarantee. ✓
Category: Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names: BRICHOS domain containing 2A | E25A | Integral membrane protein 2A | ITM2A | Protein E25 | BRICD2A
Recommended Dilution: WB: 1:500 - 1:2000
UniProt Number: O43736
NCBI GenBank: NM_004867 | NP_004858.1
SwissProt: O43736

Target

Target: Human ITM2A
Antigen Species: Human
Gene Name: ITM2A

Antibody

Host Species: Rabbit
Clonality: Polyclonal
Isotype: IgG

Immunogen

Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-263 of human ITM2A (NP_004858.1). MVKIAFNTPTAVQKEEARQDVEALLSRTVRTQILTGKELRVATQEKEGSSGRCMLTLLGLSFILAGLIVGGACIYKYFMPKSTIYRGEMCFFDSEDPANSLRGGEPNFLPVTEEADIREDDNIAIIDVPVPSFSDSDPAAIIHDFEKGMTAYLDLLLGNCYLMPLNTSIVMPPKNLVELFGKLASGRYLPQTYVVREDLVAVEEIRDVSNLGIFIYQLCNNRKSFRLRRRDLLLGFNKRAIDKCWKIRHFPNEFIVETKICQE
Immunogen Type: Fusion Protein (e.g., GST, MBP tag)