Western blot analysis of extracts from COS cells, treated with EGF (200ng/ml, 30mins), using CD18/ITGB2 (Phospho-Thr758) antibody.
ITGB2 / CD18 Antibody (phospho-Thr758)
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Enzyme-Linked Immunosorbent Assay, Immunofluorescence, Western Blot |
| Reactivity: | Human, Mouse, Rat |
| Format: | Liquid |
$365.00each
Catalog Number: LS-C822220-100
Catalog Number: LS-C822220
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- Description
- CD18 antibody LS-C822220 is an unconjugated rabbit polyclonal antibody to CD18 (ITGB2) (pThr758) from human. It is reactive with human, mouse and rat. Validated for ELISA, IF and WB.
- Application
- ELISA, IF, WB
- Reactivity
- Hu, Ms, Rt
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- CD18 antigen | CD18 | Integrin beta chain, beta 2 | ITGB2 | LCAMB | MAC-1 | MFI7 | MF17 | Integrin beta-2 | LAD | LFA-1
- Recommended Dilution: ELISA
- 1:40000
- Recommended Dilution: IF/ICC
- 1:100 - 1:500
- Recommended Dilution: WB
- 1:500 - 1:1000
- UniProt Number
- P05107
- NCBI GenBank
- NM_000211 | NP_000202.3
- SwissProt
- P05107
Target
- Target
- Human ITGB2 / CD18
- Antigen Species
- Human
- Epitope
- CD18/ITGB2 (Phospho-Thr758) antibody detects endogenous levels of CD18/ITGB2 only when phosphorylated at threonine 578. (Human: Thr758; Mouse: Thr760; Rat: Thr757)
- Gene Name
- ITGB2
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Isotype
- IgG
- Origin Species
- Human
Immunogen
- Immunogen
- Synthesized phosphopeptide derived from human CD18/ITGB2 around the phosphorylation site of threonine 758 (S-A-TP-T-T).
- Immunogen Type
- Synthetic Peptide (Linear)
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- PBS (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
- Preservative
- Yes
- Purification Method
- Affinity Purified
Storage & Handling
- Storage Conditions
- Store at -20°C for up to 1 year.
- Recommended Storage Buffer
- PBS|150mM NaCl