Extracts of CHO-T cells transfected with an insulin receptor-containing vector and left unstimulated (1) or stimulated with 50 nM insulin for 5 minutes (2-5) were resolved by SDS-PAGE on a 10% Tris-glycine gel and transferred to PVDF. The membrane was blot.
INSR / Insulin Receptor Antibody (phospho-Tyr972)
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Western Blot |
| Reactivity: | Human, Mouse, Rat |
| Format: | Liquid |
$504.00each
Catalog Number: LS-C53809-100
Catalog Number: LS-C53809
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- Description
- Insulin Receptor antibody LS-C53809 is an unconjugated rabbit polyclonal antibody to Insulin Receptor (INSR) (pTyr972) from human. It is reactive with human, mouse and rat. Validated for WB.
- Application
- WB
- Reactivity
- Hu, Ms, Rt
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- CD220 | ILP receptor | Insulin receptor | IR | HHF5 | INSR | CD220 antigen
- Recommended Dilution: WB
- 1:1000
- UniProt Number
- P06213
- NCBI GenBank
- NM_000208 | NP_000199.2
- SwissProt
- P06213
Target
- Target
- Human INSR / Insulin Receptor
- Antigen Species
- Human
- Epitope
- Human and mouse IR. Rat (100% homologous) IR has not been tested, but is expected to react. IGF1R [pY950] (75% homologous) has been shown to cross-react in some cell systems
- Gene Name
- INSR
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
Immunogen
- Immunogen
- Synthetic phosphopeptide derived from the region of the human insulin receptor that contains tyrosine 972, as numbered according to Ebina, et al. (tyrosine 960 according to Ulrich, et al.). The sequence is conserved in mouse and rat.
- Immunogen Type
- Synthetic Peptide (Linear)
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- Dulbecco's PBS (without Mg2+, Ca2+), pH 7.3 (+/- 0.1), 50% glycerol, 1.0 mg/mL BSA (IgG, protease free) as a carrier.
- Preservative
- No
- Purification Method
- Affinity Purified
Storage & Handling
- Storage Conditions
- Store at 4°C or -20°C. Avoid freeze-thaw cycles.
- Recommended Storage Buffer
- PBS