Western Blot of rabbit anti-Human IL-8 Antibody. Lane 1: Recombinant Human IL-8. Load: 50 ng per lane. Primary antibody: Rabbit anti-Human IL-8 Antibody 1:1,000 overnight at 4°C. Secondary antibody: HRP Goat-a-rabbit secondary antibody at 1:40,000 for 30 min at RT. Block: MB-070 for 30 min at RT. Predicted/Observed size: 11 kDa, 11 kDa for Human IL-8.
IL8 / Interleukin 8 Antibody
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general), Immunoprecipitation, Western Blot |
| Reactivity: | Human |
| Format: | Liquid |
$579.00each
Catalog Number: LS-C744646-100
Catalog Number: LS-C744646
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- Description
- Interleukin 8 antibody LS-C744646 is an unconjugated rabbit polyclonal antibody to human Interleukin 8 (IL8). Validated for ELISA, IHC, IP and WB.
- Application
- ELISA, IHC, IP, WB
- Reactivity
- Hu
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- 3-10C | AMCF-I | B-ENAP | C-X-C motif chemokine 8 | GCP-1 | IL-8 | IL8 | Interleukin-8 | K60 | LECT | GCP1 | LUCT | NAF | NAP1 | Neutrophil-activating factor | Interleukin 8 | Protein 3-10C | MDNCF | TSG-1 | CXCL8 | Emoctakin | LYNAP | MONAP | NAP-1 | SCYB8 | T-cell chemotactic factor
- Recommended Dilution: ELISA
- 1:1000 - 1:5000
- Recommended Dilution: IHC
- 1:400 - 1:800
- Recommended Dilution: IP
- 1:400 - 1:800
- Recommended Dilution: WB
- 1:500 - 1:2000
- UniProt Number
- P10145
- NCBI GenBank
- NM_000584 | NP_000575.1
- SwissProt
- P10145
Target
- Target
- Human IL8 / Interleukin 8
- Antigen Species
- Human
- Epitope
- The antiserum detects recombinant and native IL-8 present in body fluids and cell supernatants in ELISA. Although likely to be functional, this antiserum has not been evaluated in other assays, including neutralization and immunohistochemistry.
- Gene Name
- CXCL8
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Origin Species
- Human
Immunogen
- Immunogen
- The whole rabbit serum was prepared by repeated immunizations with recombinant human IL-8 produced in E.coli.
- Immunogen Type
- Recombinant Immunogen
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- Antiserum
- Concentration
- 75 mg/mL
- Volume
- 100 Microliter
- Preservative
- No
- Purification Method
- Unpurified (Serum / Ascites / Supernatant)
Storage & Handling
- Storage Conditions
- Store vial at -20°C or below prior to opening. Dilute 1:10 to minimize loss. Store the vial at -20°C or below after dilution. Avoid freeze-thaw cycles.