Anti-IL6 Antibody - Western Blot. Western blot of anti-IL6 antibody. Protein was resolved on a 4-20% Tris-Glycine gel by SDS-PAGE and transferred onto nitrocellulose. The blot shows detection of a band ~21 kD in size corresponding to anti-IL6 antibody. Molecular weight markers are also shown (MW). After transfer, the membrane was blocked for 30 minutes with 1% BSA-TBST. Detection occurred using peroxidase conjugated anti-Rabbit IgG (LS-C60865) secondary antibody diluted 1:40000 in blocking buffer (p/n MB-070) for 30 min at RT followed by reaction with FemtoMax chemiluminescent substrate. Image was captured using VersaDoc MP 4000 imaging system (Bio-Rad).
IL6 / Interleukin 6 Antibody
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general), Immunoprecipitation, Neutralization, Radioimmunoassay, Western Blot |
| Reactivity: | Human |
| Format: | Liquid |
$1250.00each
Catalog Number: LS-C59382-1
Catalog Number: LS-C59382
For bulk orders or custom formulations:
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- Description
- Interleukin 6 antibody LS-C59382 is an unconjugated rabbit polyclonal antibody to human Interleukin 6 (IL6). Validated for ELISA, IHC, IP, Neut, RIA and WB.
- Application
- ELISA, IHC, IP, Neut, RIA, WB
- Reactivity
- Hu
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- B-cell stimulatory factor 2 | BSF-2 | BSF2 | CTL differentiation factor | Hybridoma growth factor | IFN-beta-2 | IL6 | Interleukin-6 | Interleukin BSF-2 | IFNB2 | B-cell differentiation factor | CDF | HSF | IL-6
- Recommended Dilution: ELISA
- 1:1000 - 1:5000
- Recommended Dilution: IHC
- 1:400 - 1:800
- Recommended Dilution: IP
- 1:400 - 1:800
- Recommended Dilution: Other
- Neutralization: 1:400
- Recommended Dilution: WB
- 1:500 - 1:2000
- UniProt Number
- P05231
- NCBI GenBank
- NM_000600 | NP_000591.1
- SwissProt
- P05231
Target
- Target
- Human IL6 / Interleukin 6
- Antigen Species
- Human
- Gene Name
- IL6
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
Immunogen
- Immunogen
- This whole rabbit serum was prepared by repeated immunizations with recombinant human IL-6 produced in E.coli.
- Immunogen Type
- Recombinant Immunogen
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl
- Concentration
- 80 mg/mL
- Volume
- 1 Milliliter
- Preservative
- No
- Purification Method
- Unpurified (Serum / Ascites / Supernatant)
Storage & Handling
- Storage Conditions
- Short term: -20°C; Long term: -20°C.
- Recommended Storage Buffer
- 0.02 M Potassium Phosphate|0.15 M NaCl