Immunodetection Analysis: Representative blot from a previous lot. Lane 1, protein marker; Lane 2, recombinant protein IL-6; Lane 3, 293T lysate; Lane 4, SP2/0 lysate. The membrane blot was probed with anti-IL-6 primary antibody (1µg/ ml). Proteins were visualized using a donkey antimouse secondary antibody conjugated to IRDye 800CW detection system.
IL6 / Interleukin 6 Antibody (clone 1B1-B10-D2, Preservative Free, Biotin)
Primary AntibodyLSBio Guarantee
| Antibody: | Mouse Monoclonal |
| Applications: | Enzyme-Linked Immunosorbent Assay, Western Blot |
| Reactivity: | Human |
| Format: | Lyophilized |
$350.00each
Catalog Number: LS-C342908-50
Catalog Number: LS-C342908
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- Description
- Interleukin 6 antibody LS-C342908 is a biotin-conjugated mouse monoclonal antibody to human Interleukin 6 (IL6). Validated for ELISA and WB.
- Application
- ELISA, WB
- Reactivity
- Hu
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Monoclonal Antibodies
- Alternative Names
- B-cell stimulatory factor 2 | BSF-2 | BSF2 | CTL differentiation factor | Hybridoma growth factor | IFN-beta-2 | IL6 | Interleukin-6 | Interleukin BSF-2 | IFNB2 | B-cell differentiation factor | CDF | HSF | IL-6
- Recommended Dilution: ELISA
- 15.6 ug/ml
- Recommended Dilution: WB
- 1:1000
- UniProt Number
- P05231
- NCBI GenBank
- NM_000600 | NP_000591.1
- SwissProt
- P05231
Target
- Target
- Human IL6 / Interleukin 6
- Antigen Species
- Human
- Epitope
- The mouse anti-human IL-6 antibody specifically detects targets derived from human recombinant protein at various concentrations. Cross reactivity with mouse and rat are expected from sequence similarity. The antibody specifically binds to its immunogen and did not show any cross reactivity with unrelated antigens in ELISA. The specificity for binding to recombinant protein, cellular protein and native antigen is not defined.
- Gene Name
- IL6
Antibody
- Host Species
- Mouse
- Clonality
- Monoclonal
- Clone
- 1B1-B10-D2
- Isotype
- IgG1
Immunogen
- Immunogen
- Recombinant protein derived from IL-6.
- Immunogen Type
- Recombinant Immunogen
Format
- Conjugate/Label
- Biotin
- Format
- Lyophilized
- Formulation
- Lyophilized from PBS, No preservatives added
- Preservative
- No
- Purification Method
- Protein A/G Affinity
Storage & Handling
- Storage Conditions
- Short term: Store at 4 °C for 1 month. Long term: Store at -20°C to -70°C for up to 1 year. Avoid freeze-thaw cycles.
- Recommended Storage Buffer
- PBS