Western blot of lysate from MDA-MB-453 cell line, using IL5RA Antibody. Antibody was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug per lane.
IL5RA / CD125 Antibody (aa49-76)
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Flow Cytometry, Immunohistochemistry (general), Immunohistochemistry, Paraffin, Western Blot |
| Reactivity: | Human |
| Format: | Liquid |
$401.00each
Catalog Number: LS-C165211-400
Catalog Number: LS-C165211
For bulk orders or custom formulations:
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- Description
- CD125 antibody LS-C165211 is an unconjugated rabbit polyclonal antibody to human CD125 (IL5RA) (aa49-76). Validated for Flow, IHC and WB.
- Application
- FC, IHC, IHC-P, WB
- Reactivity
- Hu
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- CD125 | CD125 antigen | CDw125 | HSIL5R3 | IL-5R-alpha | IL5RA | Interleukin 5 receptor type 3 | Interleukin 5 receptor, alpha | IL-5 receptor subunit alpha | IL-5R subunit alpha | IL-5RA | IL5R
- Recommended Dilution: FC
- 1:10 - 1:50
- Recommended Dilution: IHC-P
- 1:50 - 1:100
- Recommended Dilution: WB
- 1:1000
- UniProt Number
- Q01344
- NCBI GenBank
- NM_000564 | NP_000555.2
- SwissProt
- Q01344
Target
- Target
- Human IL5RA / CD125
- Antigen Species
- Human
- Epitope
- This IL5RA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 49-76 amino acids from the N-terminal region of human IL5RA.
- Gene Name
- IL5RA
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
Immunogen
- Immunogen Type
- Peptide
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- PBS, 0.09% Sodium Azide
- Concentration
- 0.5 mg/mL
- Volume
- 400 Microliter
- Preservative
- Yes
- Purification Method
- Protein A Affinity
Storage & Handling
- Storage Conditions
- Maintain refrigerated at 2°C to 8°C for up to 6 months. For long term storage store at -20°C.
- Recommended Storage Buffer
- PBS