IL4R / CD124 Antibody (Extracellular Domain, clone 25463 (49/20))
Primary AntibodyLSBio Guarantee
| Antibody: | Mouse Monoclonal |
| Applications: | Enzyme-Linked Immunosorbent Assay, Flow Cytometry, Immunohistochemistry (general), Immunohistochemistry, Paraffin, Western Blot |
| Reactivity: | Human |
| Format: | Liquid |
$316.00each
Catalog Number: LS-C11209-0.1
Catalog Number: LS-C11209
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- Description
- CD124 antibody LS-C11209 is an unconjugated mouse monoclonal antibody to human CD124 (IL4R) (Extracellular Domain). Validated for ELISA, Flow, IHC and WB.
- Application
- ELISA, FC, IHC, IHC-P, WB
- Reactivity
- Hu
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Monoclonal Antibodies
- Alternative Names
- CD124 | CD124 antigen | IL-4RA | IL4R nirs variant 1 | IL4RA | IL4R | Interleukin 4 receptor | IL-4 receptor subunit alpha | IL-4R subunit alpha | IL-4R-alpha
- Recommended Dilution: ELISA
- 1:250 - 1:1000
- Recommended Dilution: FC
- 1:10 - 1:40
- Recommended Dilution: IHC-P
- 1:20 - 1:60
- Recommended Dilution: WB
- 1:125 - 1:500
- UniProt Number
- P24394
- NCBI GenBank
- NM_000418 | NP_000409.1
- SwissProt
- P24394
Target
- Target
- Human IL4R / CD124
- Antigen Species
- Human
- Epitope
- Recognizes human Interleukin-4 receptor subunit alpha, also known as CD124. CD124 is a 825 amino acid ~90kD type I single pass transmembrane glycoprotein bearing a single domain. Interleukin-4 Receptor form a heterodimeric complex with the, referred to as the IL-4 type-1 complex. The alpha subunit can also form the type-II complex through association with the IL-13 receptor alpha 1 subunit. The IL-4R complex acts as a receptor for both interleukin-4 and interleukin-13. IL-4 R is expressed by B cells, T cells, monocytes and macrophages. Is reported to block human cell surface IL-4 receptor mediated bioactivities induced by IL-4 or IL-13. An ND50 was observed at approximately 5-20 ng/ml in the presence of 0.2 ng/ml of IL-4 using the factor dependant human erythroleukemic cell line, TF-1.
- Gene Name
- IL4R
Antibody
- Host Species
- Mouse
- Clonality
- Monoclonal
- Clone
- 25463 (49/20)
- Isotype
- IgG2a
Immunogen
- Immunogen
- Sf 21-derived, recombinant human interleukin 4 receptor, extracellular domain.
- Immunogen Type
- Recombinant Immunogen
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- PBS, 0.5% trehalose
- Concentration
- 0.5 mg/mL
- Preservative
- No
- Purification Method
- Protein A Affinity
Storage & Handling
- Storage Conditions
- Store at -20°C. Store undiluted. Avoid freeze-thaw cycles. Microcentrifugation recommended if solution contains precipitate.
- Recommended Storage Buffer
- PBS