Western blot of Biotin Conjugated Rabbit anti-IL-4 Antibody. Lane 1: Human IL-4. Lane 2: none. Load: 50 ng per lane. Primary antibody: IL-4 Antibody Biotin Conjugated at 1:1000 for overnight at 4C. Secondary antibody: HRP Streptavidin secondary antibody at 1:40000 for 30 min at RT. Block: MB-070 for 30 min at RT. Predicted/Observed size: 17 kDa, 17 kDa for Human IL-4. Other band(s): none. This image was taken for the unconjugated form of this product. Other forms have not been tested.

IL4 Antibody (Biotin)

Primary AntibodyLSBio Guarantee

Antibody:	Rabbit Polyclonal
Applications:	Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general), Western Blot
Reactivity:	Human
Format:	Lyophilized

$564.00each

Catalog Number: LS-C154120-100

Unit Size

Quantity

For bulk orders or custom formulations:

Overview

Description: IL4 antibody LS-C154120 is a biotin-conjugated rabbit polyclonal antibody to human IL4. Validated for ELISA, IHC and WB.
Application: ELISA, IHC, WB
Reactivity: Hu
Intended Use: Research Use Only
Guarantees: This antibody carries the LSBio 100% Guarantee. ✓
Category: Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names: B cell growth factor 1 | B-cell stimulatory factor 1 | B_cell stimulatory factor 1 | BCGF1 | BSF-1 | Binetrakin | BCGF-1 | Interleukin 4 | Interleukin-4 | IL4 | Pitrakinra | BSF1 | IL-4
Recommended Dilution: ELISA: 1:20000 - 1:100000
Recommended Dilution: IHC: 1:1000 - 1:5000
Recommended Dilution: WB: 1:2000 - 1:10000
UniProt Number: P05112
NCBI GenBank: NM_000589 | NP_000580.1
SwissProt: P05112

Target

Target: Human IL4
Antigen Species: Human
Epitope: This antibody will recognize both native and recombinant human IL-4 in cell supernatants and certain body fluids. A control of similarly diluted normal rabbit IgG is recommended.
Gene Name: IL4

Antibody

Host Species: Rabbit
Clonality: Polyclonal
Isotype: IgG

Immunogen

Immunogen: This purified antibody was prepared from whole rabbit serum produced by repeated immunizations with full length recombinant human IL-4 protein.
Immunogen Type: Full-Length Recombinant Protein

Format

Conjugate/Label: Biotin
Format: Lyophilized
Formulation: Lyophilized from 10 mg/mL BSA (Protease and Ig free), 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Concentration: 0.8 mg/mL
Preservative: Yes
Purification Method: Ion Exchange Chromatography

Storage & Handling

Storage Conditions: Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
Recommended Storage Buffer: 0.02 M Potassium Phosphate|0.15 M NaCl