Western blot using the Protein-A Purified anti-bovine IL-1F5 antibody shows detection of recombinant bovine IL-1F5 at 17.1 kDa (arrowhead) raised in yeast. The identity of the band at 36 kDa is not known. The protein was purified and resolved by SDS-PAGE, then transferred to PVDF membrane. Membrane was blocked with 3% BSA (BSA-30, diluted 1:10), and probed with 1 µg/mL primary antibody overnight at 4°C. After washing, membrane was probed with for 45 min at room temperature.
IL36RN / IL1F5 Antibody (Liquid)
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Enzyme-Linked Immunosorbent Assay, Western Blot |
| Reactivity: | Bovine |
| Format: | Liquid |
$311.00each
Catalog Number: LS-C745207-25
Catalog Number: LS-C745207
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- Description
- IL1F5 antibody LS-C745207 is an unconjugated rabbit polyclonal antibody to bovine IL1F5 (IL36RN). Validated for ELISA and WB.
- Application
- ELISA, WB
- Reactivity
- Bov
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- FIL1 | FIL1D | IL-1 delta | IL-1 related protein 3 | IL-1HY1 | IL-1RP3 | IL-1-related protein 3 | IL1F5 | IL1RP3 | IL1L1 | IL36RA | Interleukin-1 delta | Family of interleukin 1-delta | FIL1 delta | IL-1L1 | IL-1ra homolog 1 | IL36RN | Interleukin-1 family member 5 | Interleukin-1-like protein 1 | FIL1(DELTA) | IL-1F5 | IL1HY1 | Interleukin-1 HY1 | PSORP
- Recommended Dilution: ELISA
- 1:8000
- Recommended Dilution: WB
- 1 µg/ml
- UniProt Number
- Q9UBH0
- NCBI GenBank
- NM_012275 | NP_036407.1
- SwissProt
- Q9UBH0
Target
- Target
- Bovine IL36RN / IL1F5
- Antigen Species
- Bovine
- Epitope
- This antibody is specific for bovine IL-1F5 protein. A BLAST analysis was used to suggest cross-reactivity with IL-1F5 from dog, panda, and ferret based on 90-92% homology with the immunizing sequence.
- Gene Name
- IL36RN
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Isotype
- IgG
- Origin Species
- Human
Immunogen
- Immunogen
- This protein-A purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a recombinant protein raised in yeast, corresponding to the 155 amino acids of the mature bovine IL-1F5 protein.
- Immunogen Type
- Recombinant Immunogen
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
- Concentration
- 1 mg/mL
- Volume
- 25 Microliter
- Preservative
- Yes
- Purification Method
- Protein A Affinity
Storage & Handling
- Storage Conditions
- Store vial at -20°C or below prior to opening. Dilute 1:10 to minimize loss. Store the vial at -20°C or below after dilution. Avoid freeze-thaw cycles.
- Recommended Storage Buffer
- 0.02 M Potassium Phosphate|0.15 M NaCl