IHC analysis of IL36 alpha using anti-IL36 alpha antibody. IL36 alpha was detected in paraffin-embedded section of human intestinal cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2?g/ml rabbit anti-IL36 alpha Antibody overnight at 4?C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37?C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.

IL36A Antibody

Primary AntibodyLSBio Guarantee

Antibody:	Rabbit Polyclonal
Applications:	Enzyme-Linked Immunosorbent Assay, Immunohistochemistry, Paraffin, Western Blot
Reactivity:	Human, Mouse, Rat
Format:	Lyophilized

$480.00each

Catalog Number: LS-C756606-100

Unit Size

Quantity

For bulk orders or custom formulations:

Overview

Description: IL36A antibody LS-C756606 is an unconjugated rabbit polyclonal antibody to IL36A from human. It is reactive with human, mouse and rat. Validated for ELISA, IHC and WB.
Application: ELISA, IHC-P, WB
Reactivity: Hu, Ms, Rt
Intended Use: Research Use Only
Guarantees: This antibody carries the LSBio 100% Guarantee. ✓
Category: Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names: FIL1 | FIL1 epsilon | IL-1F6 | IL1(EPSILON) | IL1E | IL1F6 | Interleukin 1, epsilon | Interleukin 36, alpha | Interleukin-1 epsilon | Interleukin-1 family member 6 | FIL1(EPSILON) | FIL1E | IL-1 epsilon | IL-1F6 (FIL-1-epsilon) | IL36A | Interleukin-36 alpha
Recommended Dilution: ELISA: 1 - 5 ug/ml
Recommended Dilution: IHC-P: 0.5 - 1 µg/ml
Recommended Dilution: WB: 0.1 - 0.5 µg/ml
UniProt Number: Q9UHA7
NCBI GenBank: NM_014440 | NP_055255.1
SwissProt: Q9UHA7

Target

Target: Human IL36A
Antigen Species: Human
Epitope: No cross reactivity with other proteins.
Gene Name: IL36A

Antibody

Host Species: Rabbit
Clonality: Polyclonal
Isotype: IgG
Origin Species: Human

Immunogen

Immunogen: E. coli-derived human IL36 alpha recombinant protein (Position: K6-D151).
Immunogen Type: Recombinant Immunogen

Format

Conjugate/Label: Unconjugated
Format: Lyophilized
Formulation: Lyophilized from 0.2mg Na2HPO4, 0.9mg NaCl, 0.05mg sodium azide, 4mg Trehalose
Preservative: Yes
Purification Method: Affinity Purified

Storage & Handling

Storage Conditions: After reconstitution, may be stored at 4°C for 1 month. For long-term storage and to avoid freeze-thaw cycles, aliquot and store at -20°C.
Recommended Storage Buffer: NaCl