Western blot analysis of extracts from LOVO cells, using IL-2Ra/CD25 (Phospho-Ser268) antibody.
IL2RA / CD25 Antibody (phospho-Ser268)
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Enzyme-Linked Immunosorbent Assay, Immunofluorescence, Western Blot |
| Reactivity: | Human, Mouse |
| Format: | Liquid |
$365.00each
Catalog Number: LS-C822740-100
Catalog Number: LS-C822740
For bulk orders or custom formulations:
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- Description
- CD25 antibody LS-C822740 is an unconjugated rabbit polyclonal antibody to CD25 (IL2RA) (pSer268) from human. It is reactive with human and mouse. Validated for ELISA, IF and WB.
- Application
- ELISA, IF, WB
- Reactivity
- Hu, Ms
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- CD25 antigen | CD25 | IDDM10 | IL-2 receptor subunit alpha | IL2R | Interleukin 2 receptor, alpha | IL-2-RA | IL2-RA | IL2RA | TCGFR | IL-2R subunit alpha | p55 | TAC antigen
- Recommended Dilution: ELISA
- 1:10000
- Recommended Dilution: IF/ICC
- 1:100 - 1:500
- Recommended Dilution: WB
- 1:500 - 1:1000
- UniProt Number
- P01589
- NCBI GenBank
- NM_000417 | NP_000408.1
- SwissProt
- P01589
Target
- Target
- Human IL2RA / CD25
- Antigen Species
- Human
- Epitope
- IL-2RAlpha/CD25 (Phospho-Ser268) antibody detects endogenous levels of IL-2RAlpha/CD25 only when phosphorylated at serine 268. (Human: Ser268; Mouse: Ser264)
- Gene Name
- IL2RA
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Isotype
- IgG
- Origin Species
- Human
Immunogen
- Immunogen
- Synthesized phosphopeptide derived from human IL-2RAlpha/CD25 around the phosphorylation site of serine 268 (R-K-SP-R-R).
- Immunogen Type
- Synthetic Peptide (Linear)
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- PBS (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
- Preservative
- Yes
- Purification Method
- Affinity Purified
Storage & Handling
- Storage Conditions
- Store at -20°C for up to 1 year.
- Recommended Storage Buffer
- PBS|150mM NaCl