IHC on rat spleens.
IL2RA / CD25 Antibody (IL-2R alpha (CD25), clone Basiliximab)
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Recombinant Monoclonal |
| Applications: | Blocking / Competition Assay, Flow Cytometry, Immunofluorescence, Immunohistochemistry (general), Western Blot |
| Reactivity: | Cynomolgus Monkey, Human, Rhesus Macaque |
| Format: | Liquid |
$551.00each
Catalog Number: LS-C777504-0.2
Catalog Number: LS-C777504
For bulk orders or custom formulations:
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- Description
- This IL2RA / CD25 antibody is an unconjugated Rabbit/Human chimeric recombinant monoclonal antibody. Validated for Block, Flo, IF, IHC, and WB.
- Application
- Blocking, FC, IF, IHC, WB
- Reactivity
- Cyno, Hu, Rhes
- Intended Use
- RUO
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Recombinant Antibodies
- Alternative Names
- CD25 antigen | CD25 | IDDM10 | IL-2 receptor subunit alpha | IL2R | Interleukin 2 receptor, alpha | IL-2-RA | IL2-RA | IL2RA | TCGFR | IL-2R subunit alpha | p55 | TAC antigen
- Recommended Dilution: IF/ICC
- 5 µg/ml
- Recommended Dilution: IHC
- 5 µg/ml
- UniProt Number
- P01589
- NCBI GenBank
- NM_000417, NP_000408.1
- SwissProt
- P01589
Target
- Target
- Human IL2RA / CD25
- Antigen Species
- Hu
- Epitope
- Basiliximab recognises the human IL-2R and binds to the epitope (116)ERIYHFV(122) within the extracellular domain of the CD25 subunit - binding is abolished when 2 or more residues within the epitope are mutated. This epitope overlaps with the interaction site of CD25 and IL-2. CD25 is found in the high-affinity IL-2R ( alpha beta gamma c) - T cells only express CD25 upon activation. IL-2 binding to IL-2R on T cells modulates proliferation and differentiation.
- Gene Name
- IL2RA
Antibody
- Host Species
- Rb
- Clonality
- Recombinant Monoclonal
- Clone
- Basiliximab
- Isotype
- IgG
- Light Chain
- κ
- Origin Species
- Hu
Immunogen
- Immunogen
- Basiliximab was prepared by immunizing BALB/c mice with CTC 16 cells.
- Immunogen Type
- Cell
Format
- Conjugate/Label
- Unconj
- Format
- Liquid
- Formulation
- PBS, 0.02% ProClin™ 300.
- Concentration
- 1 mg/mL
- Preservative
- Yes
- Purification Method
- Prot A
Storage & Handling
- Storage Conditions
- Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
- Recommended Storage Buffer
- PBS