LSBio
IL2RA / CD25 Antibody (clone OX-39)

IL2RA / CD25 Antibody (clone OX-39)

Primary AntibodyLSBio Guarantee
Antibody:Mouse Monoclonal
Applications:Enzyme-Linked Immunosorbent Assay, Flow Cytometry, Immunohistochemistry (general), Immunohistochemistry, Frozen, Immunohistochemistry, Paraffin, Immunoprecipitation
Reactivity:Rat
Format:Liquid
See all Rat IL2RA / CD25 antibodies →
$296.00each
Catalog Number: LS-C45048-0.1

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Overview

Description
CD25 antibody LS-C45048 is an unconjugated mouse monoclonal antibody to rat CD25 (IL2RA). Validated for ELISA, Flow, IHC and IP.
Application
ELISA, FC, IHC, IHC-F, IHC-P, IP
Reactivity
Rt
Intended Use
Research Use Only
Guarantees
This antibody carries the LSBio 100% Guarantee.
Category
Antibodies > Primary Antibodies > Monoclonal Antibodies
Alternative Names
CD25 antigen | CD25 | IDDM10 | IL-2 receptor subunit alpha | IL2R | Interleukin 2 receptor, alpha | IL-2-RA | IL2-RA | IL2RA | TCGFR | IL-2R subunit alpha | p55 | TAC antigen
Recommended Dilution: FC
1:10 - 1:50
UniProt Number
P01589
SwissProt
P01589

Target

Target
Rat IL2RA / CD25
Antigen Species
Rat
Epitope
Recognizes the alpha chain of rat CD25, otherwise known as IL-2 receptor alpha, a 55kD type I membrane glycoprotein, expressed by activated T cells but not resting lymphocytes. CD25 is also expressed by dendritic cells found in the thymus medulla. *This clone has been described reacting with paraffin-embedded material following PLP fixation (periodate-lysine-paraformaldehyde). Clone OX-39 has been shown to weakly inhibit the binding of IL-2 to Con-A stimulated spleen blasts. XXX recommend the use of MCA273EL for this purpose.
Gene Name
IL2RA

Antibody

Host Species
Mouse
Clonality
Monoclonal
Clone
OX-39
Isotype
IgG1

Immunogen

Immunogen
Stimulated Rat T cells.
Immunogen Type
Cell-Based Immunization

Format

Conjugate/Label
Unconjugated
Format
Liquid
Formulation
PBS, 0.09% Sodium Azide
Concentration
1 mg/mL
Preservative
Yes
Purification Method
Protein G Affinity

Storage & Handling

Storage Conditions
Store at 4°C or at -20°C. Store undiluted. Avoid freeze-thaw cycles. Microcentrifugation recommended if solution contains precipitate.
Recommended Storage Buffer
PBS

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