Flow cytometric analysis of Human IL2 expression on human peripheral blood lymphocytes. Human peripheral blood mononulcear cells were stimulated for 4-6 hours with PMA and Ionomycin in the presence of GolgiPlug. The cells were treated according to manufacturer's manual (BD Pharmingen Cat. No. 554714), stained with PE-conjugated anti-Human IL2 and FITC-conjugated anti-Human CD3. The dot plots were derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes.