Western Blot of Rabbit Anti-Human IL-1 alpha Antibody. Lane 1: Opal Prestained Molecular Weight Ladder Lane 2: IL-1 alpha 50ng. Lane 3: MEF WC lysate 10ng. Lane 4: MEF LPS stimulated 10ng.BlockOut Buffer for 30min at RT. Primary Antibody: Anti-IL-1a 1µg/mL overnight at 4°C. Secondary Antibody: Goat anti-Rabbit HRP at 1:70, 000) for 30min at RT.
IL1A / IL-1 Alpha Antibody
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Enzyme-Linked Immunosorbent Assay, Flow Cytometry, Immunohistochemistry (general), Neutralization, Western Blot |
| Reactivity: | Human |
| Format: | Liquid |
$1250.00each
Catalog Number: LS-C18826-1
Catalog Number: LS-C18826
For bulk orders or custom formulations:
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- Description
- IL-1 Alpha antibody LS-C18826 is an unconjugated rabbit polyclonal antibody to human IL-1 Alpha (IL1A). Validated for ELISA, Flow, IHC, Neut and WB.
- Application
- ELISA, FC, IHC, Neut, WB
- Reactivity
- Hu
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- Hematopoietin-1 | IL-1 alpha | IL1 | IL1-ALPHA | IL1 Alpha | Interleukin-1 alpha | IL1A | IL-1A | IL1F1 | Pro-interleukin-1-alpha | Preinterleukin 1 alpha | Interleukin 1, alpha
- Recommended Dilution: ELISA
- 1:1000 - 1:5000
- Recommended Dilution: WB
- 1:500 - 1:2000
- UniProt Number
- P01583
- NCBI GenBank
- NM_000575 | NP_000566.3
- SwissProt
- P01583
Target
- Target
- Human IL1A / IL-1 Alpha
- Antigen Species
- Human
- Gene Name
- IL1A
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Isotype
- IgG
Immunogen
- Immunogen
- The whole rabbit serum used to produce this IgG fraction antibody was prepared by repeated immunizations with recombinant human IL-1a produced in E.coli. The MW of the recombinant IL-1a was 17,000. This is the cleavage site generated by the IL-1b converting enzyme (ICE, capase-1).
- Immunogen Type
- Recombinant Immunogen
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
- Concentration
- 1 mg/mL
- Preservative
- Yes
- Purification Method
- Unpurified (Serum / Ascites / Supernatant)
Storage & Handling
- Storage Conditions
- Store vial at -20°C prior to opening. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20°C or below. Avoid freeze-thaw cycles.
- Recommended Storage Buffer
- 0.02 M Potassium Phosphate|0.15 M NaCl