Western blot analysis of extracts of various cell lines, using IL12B antibody at 1:3000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 20s.
IL12B / IL12 p40 Antibody
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Western Blot |
| Reactivity: | Human |
| Format: | Liquid |
$281.00each
Catalog Number: LS-C748087-20
Catalog Number: LS-C748087
For bulk orders or custom formulations:
Request a QuoteOverview
- Description
- IL12 p40 antibody LS-C748087 is an unconjugated rabbit polyclonal antibody to human IL12 p40 (IL12B). Validated for WB.
- Application
- WB
- Reactivity
- Hu
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- CLMF p40 | IL-12 p40 | IL-12 subunit p40 | Interleukin-12 beta chain | Interleukin-12 subunit beta | IL12, subunit p40 | IL12B | Interleukinl-12 | Interleukin 12, p40 | NKSF | CLMF | CLMF2 | IL-12B | NKSF2
- Recommended Dilution: WB
- 1:1000 - 1:2000
- UniProt Number
- P29460
- NCBI GenBank
- NM_002187 | NP_002178.2
- SwissProt
- P29460
Target
- Target
- Human IL12B / IL12 p40
- Antigen Species
- Human
- Gene Name
- IL12B
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Isotype
- IgG
- Origin Species
- Human
Immunogen
- Immunogen
- Recombinant fusion protein containing a sequence corresponding to amino acids 23-328 of human IL12B (NP_002178.2). IWELKKDVYVVELDWYPDAPGEMVVLTCDTPEEDGITWTLDQSSEVLGSGKTLTIQVKEFGDAGQYTCHKGGEVLSHSLLLLHKKEDGIWSTDILKDQKEPKNKTFLRCEAKNYSGRFTCWWLTTISTDLTFSVKSSRGSSDPQGVTCGAATLSAERVRGDNKEYEYSVECQEDSACPAAEESLPIEVMVDAVHKLKYENYTSSFFIRDIIKPDPPKNLQLKPLKNSRQVEVSWEYPDTWSTPHSYFSLTFCVQVQGKSKREKKDRVFTDKTSATVICRKNASISVRAQDRYYSSSWSEWASVPCS
- Immunogen Type
- Fusion Protein (e.g., GST, MBP tag)
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- PBS, pH 7.3, 0.02% Sodium Azide, 50% Glycerol
- Volume
- 20 Microliter
- Preservative
- Yes
- Purification Method
- Affinity Purified
Storage & Handling
- Storage Conditions
- Store at -20°C. Avoid freeze-thaw cycles.
- Recommended Storage Buffer
- PBS