Western Blot of rabbit anti-NEMO antibody. Lane 1: Opal Pre-stained ladder Lane 2: Recombinant NEMO protein. Load: 175 ng per lane. Primary antibody: NEMO antibody at 1:1,000 for overnight at 4°C. Secondary antibody: Peroxidase rabbit secondary antibody at 1:70,000 for 30 min at RT. Blocking Buffer: MB-070 for 30 min at RT. Predicted/Observed size: 55 and 47 kDa for NEMO.
IKBKG / NEMO / IKK Gamma Antibody
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Enzyme-Linked Immunosorbent Assay, Immunoprecipitation, Western Blot |
| Reactivity: | Human |
| Format: | Liquid |
$311.00each
Catalog Number: LS-C745002-25
Catalog Number: LS-C745002
For bulk orders or custom formulations:
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- Description
- IKK Gamma antibody LS-C745002 is an unconjugated rabbit polyclonal antibody to human IKK Gamma (IKBKG / NEMO). Validated for ELISA, IP and WB.
- Application
- ELISA, IP, WB
- Reactivity
- Hu
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- AMCBX1 | FIP3 | Fip3p | IkappaB kinase gamma | IKKgamma | IKK-gamma | NEMO | NF-kappa-B essential modifier | I-kappa-B kinase gamma-subunit | I-kappa-B kinase subunit gamma | IkB kinase gamma subunit | IKBKG | IKKAP1 | Incontinentia pigmenti | IP2 | IPD2 | NF-kappa-B essential modulator | ZC2HC9 | FIP-3 | IkB kinase subunit gamma | IKKG | IP1 | NFkappaB essential modulator
- Recommended Dilution: ELISA
- 1:50000 - 1:400000
- Recommended Dilution: WB
- 1 µg/ml
- UniProt Number
- Q9Y6K9
- NCBI GenBank
- NM_003639 | NP_003630.1
- SwissProt
- Q9Y6K9
Target
- Target
- Human IKBKG / NEMO / IKK Gamma
- Antigen Species
- Human
- Epitope
- This antibody detects human NEMO. Cross reactivity with NEMO from other sources is unknown.
- Gene Name
- IKBKG
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Isotype
- IgG
- Origin Species
- Human
Immunogen
- Immunogen
- Anti-NEMO was affinity purified from whole rabbit serum prepared by repeated immunizations with a recombinant protein of human NEMO.
- Immunogen Type
- Recombinant Immunogen
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
- Concentration
- 1.1 mg/mL
- Volume
- 25 Microliter
- Preservative
- Yes
- Purification Method
- Ion Exchange Chromatography
Storage & Handling
- Storage Conditions
- Store vial at -20°C prior to opening. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20°C or below. Avoid freeze-thaw cycles.
- Recommended Storage Buffer
- 0.02 M Potassium Phosphate|0.15 M NaCl